Functional similarity of HIV-I rev and HTLV-I rex proteins: Identification of a new nucleolar-targeting signal in rev protein

Satoshi Kubota, Haruhiko Siomi, Takumi Satoh, Sei ichi Endo, Masatoshi Maki, Masakazu Hatanaka

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105 Citations (Scopus)


We have tested the functional compatibility between rev protein of human immunodeficiency virus type I (HIV-I) and rex protein of human T-cell lymphotropic virus type I (HTLV-I). Each protein recognized the other's cis-acting sequence, albeit at reduced levels. Both proteins localize predominantly in the nucleolus. We have identified a new nucleolar-targeting signal in rev protein, which was homologous to that of rex protein. The sequence [35-RQARRNRRRRWRERQR-50] in rev protein, when fused to the amino-terminus of β-galactosidase, directed the hybrid protein to the cell nucleolus. A deletion mutant which lacks several amino acid residues within the signal failed to function in the CAT assay system. These results demonstrate that the nucleolar targeting signals are essential for the functions of Rev and Rex.

Original languageEnglish
Pages (from-to)963-970
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 1989 Aug 15


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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