Fundamental study on photodynamic therapy for atrial fibrillation: Effect of photosensitization reaction parameters on myocardial necrosis in vitro

Emiyu Ogawa, Arisa Ito, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We studied necrotic cell death effect on myocardial cells with photosensitizer existed outside the cells varying photosensitization reaction parameters widely in vitro. We have developed non-thermal ablator with the application of photosensitization reaction for atrial fibrillation. Since laser irradiation is applied shortly after photosensitizer injection, the photosensitization reaction is induced outside the cells. The interaction for the myocardial cells by the photosensitization reaction is not well understood yet on various photosensitization reaction parameters. Rat myocardial cells were cultured in 96 well plates for 7 days. The photosensitization reaction was applied with talaporfin sodium (NPe6) and the semiconductor laser of 663nm wavelength. The average drug light interval was set 8 mins. The photosensitizer concentration and radiant exposure were varied from 5 to 40 μg/ml and 1.2 to 60 J/cm 2, respectively. The well bottom was irradiated by the red laser with irradiance of 293 mW/cm 2. The photosensitizer fluorescence was monitored during the photosensitization reaction. Alive cell rate was measured by WST assay after 2 hours from the irradiation. In the case of the photosensitizer concentration of 10 μg/ml, the myocardial cells were almost alive even thought 60 J/cm 2 in the radiant exposure was applied. In the 15 μg/ml case, the alive cell rate was almost linear relation to the photosensitizer concentration and radiant exposure. We obtained that the threshold for myocardial cell necrosis on the photosensitizer concentration was around 15 μg/ml with 20 J/cm 2 in the radiant exposure. This threshold on the photosensitizer concentration was similar to the reported threshold for cancer therapy.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXIII
DOIs
Publication statusPublished - 2012 Apr 2
EventOptical Interactions with Tissue and Cells XXIII - San Francisco, CA, United States
Duration: 2012 Jan 232012 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8221
ISSN (Print)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXIII
CountryUnited States
CitySan Francisco, CA
Period12/1/2312/1/25

Keywords

  • NPe6
  • Talaporfin sodium
  • cardiomyocyte
  • cell

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Fundamental study on photodynamic therapy for atrial fibrillation: Effect of photosensitization reaction parameters on myocardial necrosis in vitro'. Together they form a unique fingerprint.

  • Cite this

    Ogawa, E., Ito, A., & Arai, T. (2012). Fundamental study on photodynamic therapy for atrial fibrillation: Effect of photosensitization reaction parameters on myocardial necrosis in vitro. In Optical Interactions with Tissue and Cells XXIII [822107] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8221). https://doi.org/10.1117/12.907176