Fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis

Atsushi Saito, Satoru Inagaki, Ryuta Kimizuka, Katsuji Okuda, Yasuo Hosaka, Taneaki Nakagawa, Kazuyuki Ishihara

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Invasion by Porphyromonas gingivalis has been proposed as a possible mechanism of pathogenesis in periodontal and cardiovascular diseases. Porphyromonas gingivalis have direct access to the systemic circulation and endothelium in periodontitis patients by transient bacteremia. Periodontitis can be described as one of the predominant polymicrobial infections of humans. In the present study, P. gingivalis strains were tested for their ability to invade a human gingival epithelial cell line (Ca9-22) and human aortic endothelial cells in coinfection with Fusobacterium nucleatum using antibiotic protection assays. Coinfection with F. nucleatum resulted in 2-20-fold increase in the invasion of host cells by P. gingivalis strains. The invasive abilities of P. gingivalis strains were significantly greater when incubated with a F. nucleatum clinical isolate (which possesses strong biofilm-forming ability), than when incubated with a F. nucleatum-type strain. In inhibition assays with metabolic inhibitors, a difference in inhibition profiles was observed between mono- and polymicrobial infections. Collectively, our results suggest that F. nucleatum facilitates invasion of host cells by P. gingivalis. Investigations of polymicrobial infection of host cells should improve our understanding of the role of P. gingivalis in periodontal infection and proatherogenic mechanisms.

Original languageEnglish
Pages (from-to)349-355
Number of pages7
JournalFEMS Immunology and Medical Microbiology
Volume54
Issue number3
DOIs
Publication statusPublished - 2008 Dec

Fingerprint

Fusobacterium nucleatum
Porphyromonas gingivalis
Endothelial Cells
Coinfection
Periodontitis
Periodontal Diseases
Biofilms
Bacteremia
Endothelium
Cardiovascular Diseases
Epithelial Cells
Anti-Bacterial Agents
Cell Line
Infection

Keywords

  • Bacterial invasion
  • Endothelial
  • Fusobacterium nucleatum
  • Gingival epithelial
  • Polymicrobial infection
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology

Cite this

Fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis. / Saito, Atsushi; Inagaki, Satoru; Kimizuka, Ryuta; Okuda, Katsuji; Hosaka, Yasuo; Nakagawa, Taneaki; Ishihara, Kazuyuki.

In: FEMS Immunology and Medical Microbiology, Vol. 54, No. 3, 12.2008, p. 349-355.

Research output: Contribution to journalArticle

Saito, Atsushi ; Inagaki, Satoru ; Kimizuka, Ryuta ; Okuda, Katsuji ; Hosaka, Yasuo ; Nakagawa, Taneaki ; Ishihara, Kazuyuki. / Fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis. In: FEMS Immunology and Medical Microbiology. 2008 ; Vol. 54, No. 3. pp. 349-355.
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