Fusobacterium nucleatum in Colorectal Cancer Relates to Immune Response Differentially by Tumor Microsatellite Instability Status

Tsuyoshi Hamada, Xuehong Zhang, Kosuke Mima, Susan Bullman, Yasutaka Sukawa, Jonathan A. Nowak, Keisuke Kosumi, Yohei Masugi, Tyler S. Twombly, Yin Cao, Mingyang Song, Li Liu, Annacarolina da Silva, Yan Shi, Mancang Gu, Wanwan Li, Hideo Koh, Katsuhiko Nosho, Kentaro Inamura, Na Na Keum & 12 others Kana Wu, Jeffrey A. Meyerhardt, Aleksandar D. Kostic, Curtis Huttenhower, Wendy S. Garrett, Matthew Meyerson, Edward L. Giovannucci, Andrew T. Chan, Charles S. Fuchs, Reiko Nishihara, Marios Giannakis, Shuji Ogino

Research output: Contribution to journalArticle

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Abstract

The presence of Fusobacterium nucleatum (F. nucleatum) in colorectal carcinoma tissue has been associated with microsatellite instability (MSI), lower-level T-cell infiltrates, and poor clinical outcomes. Considering differences in the tumor-immune microenvironment between MSI-high and non-MSI-high carcinomas, we hypothesized that the association of F. nucleatum with immune response might differ by tumor MSI status. Using samples from 1,041 rectal and colon cancer patients within the Nurses' Health Study and Health Professionals Follow-up Study, we measured F. nucleatum DNA in tumor tissue by a quantitative polymerase chain reaction assay. Multivariable logistic regression models were used to examine the association between F. nucleatum status and histopathologic lymphocytic reactions or density of CD3+ cells, CD8+ cells, CD45RO (PTPRC)+ cells, or FOXP3+ cells in strata of tumor MSI status. We adjusted for potential confounders, including CpG island methylator phenotype; LINE-1 methylation; and KRAS, BRAF, and PIK3CA mutations. The association of F. nucleatum with tumor-infiltrating lymphocytes (TIL) and intratumoral periglandular reaction differed by tumor MSI status (Pinteraction = 0.002). The presence of F. nucleatum was negatively associated with TIL in MSI-high tumors [multivariable odds ratio (OR), 0.45; 95% confidence interval (CI), 0.22-0.92], but positively associated with TIL in non-MSI-high tumors (multivariable OR 1.91; 95% CI, 1.12-3.25). No significant differential association was observed for peritumoral lymphocytic reaction, Crohn-like lymphoid reaction, or T-cell densities. In conclusion, the association of F. nucleatum with immune response to colorectal carcinoma differs by tumor MSI status, suggesting that F. nucleatum and MSI status interact to affect antitumor immune reactions. Cancer Immunol Res; 6(11); 1327-36.

Original languageEnglish
Pages (from-to)1327-1336
Number of pages10
JournalCancer immunology research
Volume6
Issue number11
DOIs
Publication statusPublished - 2018 Nov 1
Externally publishedYes

Fingerprint

Fusobacterium nucleatum
Microsatellite Instability
Colorectal Neoplasms
Tumor-Infiltrating Lymphocytes
Neoplasms
Cell Count
Logistic Models
Odds Ratio
Confidence Intervals
T-Lymphocytes
CpG Islands
Tumor Microenvironment
Health
Rectal Neoplasms
Colonic Neoplasms
Methylation
Nurses
Carcinoma
Phenotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology
  • Cancer Research

Cite this

Fusobacterium nucleatum in Colorectal Cancer Relates to Immune Response Differentially by Tumor Microsatellite Instability Status. / Hamada, Tsuyoshi; Zhang, Xuehong; Mima, Kosuke; Bullman, Susan; Sukawa, Yasutaka; Nowak, Jonathan A.; Kosumi, Keisuke; Masugi, Yohei; Twombly, Tyler S.; Cao, Yin; Song, Mingyang; Liu, Li; da Silva, Annacarolina; Shi, Yan; Gu, Mancang; Li, Wanwan; Koh, Hideo; Nosho, Katsuhiko; Inamura, Kentaro; Keum, Na Na; Wu, Kana; Meyerhardt, Jeffrey A.; Kostic, Aleksandar D.; Huttenhower, Curtis; Garrett, Wendy S.; Meyerson, Matthew; Giovannucci, Edward L.; Chan, Andrew T.; Fuchs, Charles S.; Nishihara, Reiko; Giannakis, Marios; Ogino, Shuji.

In: Cancer immunology research, Vol. 6, No. 11, 01.11.2018, p. 1327-1336.

Research output: Contribution to journalArticle

Hamada, T, Zhang, X, Mima, K, Bullman, S, Sukawa, Y, Nowak, JA, Kosumi, K, Masugi, Y, Twombly, TS, Cao, Y, Song, M, Liu, L, da Silva, A, Shi, Y, Gu, M, Li, W, Koh, H, Nosho, K, Inamura, K, Keum, NN, Wu, K, Meyerhardt, JA, Kostic, AD, Huttenhower, C, Garrett, WS, Meyerson, M, Giovannucci, EL, Chan, AT, Fuchs, CS, Nishihara, R, Giannakis, M & Ogino, S 2018, 'Fusobacterium nucleatum in Colorectal Cancer Relates to Immune Response Differentially by Tumor Microsatellite Instability Status', Cancer immunology research, vol. 6, no. 11, pp. 1327-1336. https://doi.org/10.1158/2326-6066.CIR-18-0174
Hamada, Tsuyoshi ; Zhang, Xuehong ; Mima, Kosuke ; Bullman, Susan ; Sukawa, Yasutaka ; Nowak, Jonathan A. ; Kosumi, Keisuke ; Masugi, Yohei ; Twombly, Tyler S. ; Cao, Yin ; Song, Mingyang ; Liu, Li ; da Silva, Annacarolina ; Shi, Yan ; Gu, Mancang ; Li, Wanwan ; Koh, Hideo ; Nosho, Katsuhiko ; Inamura, Kentaro ; Keum, Na Na ; Wu, Kana ; Meyerhardt, Jeffrey A. ; Kostic, Aleksandar D. ; Huttenhower, Curtis ; Garrett, Wendy S. ; Meyerson, Matthew ; Giovannucci, Edward L. ; Chan, Andrew T. ; Fuchs, Charles S. ; Nishihara, Reiko ; Giannakis, Marios ; Ogino, Shuji. / Fusobacterium nucleatum in Colorectal Cancer Relates to Immune Response Differentially by Tumor Microsatellite Instability Status. In: Cancer immunology research. 2018 ; Vol. 6, No. 11. pp. 1327-1336.
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abstract = "The presence of Fusobacterium nucleatum (F. nucleatum) in colorectal carcinoma tissue has been associated with microsatellite instability (MSI), lower-level T-cell infiltrates, and poor clinical outcomes. Considering differences in the tumor-immune microenvironment between MSI-high and non-MSI-high carcinomas, we hypothesized that the association of F. nucleatum with immune response might differ by tumor MSI status. Using samples from 1,041 rectal and colon cancer patients within the Nurses' Health Study and Health Professionals Follow-up Study, we measured F. nucleatum DNA in tumor tissue by a quantitative polymerase chain reaction assay. Multivariable logistic regression models were used to examine the association between F. nucleatum status and histopathologic lymphocytic reactions or density of CD3+ cells, CD8+ cells, CD45RO (PTPRC)+ cells, or FOXP3+ cells in strata of tumor MSI status. We adjusted for potential confounders, including CpG island methylator phenotype; LINE-1 methylation; and KRAS, BRAF, and PIK3CA mutations. The association of F. nucleatum with tumor-infiltrating lymphocytes (TIL) and intratumoral periglandular reaction differed by tumor MSI status (Pinteraction = 0.002). The presence of F. nucleatum was negatively associated with TIL in MSI-high tumors [multivariable odds ratio (OR), 0.45; 95{\%} confidence interval (CI), 0.22-0.92], but positively associated with TIL in non-MSI-high tumors (multivariable OR 1.91; 95{\%} CI, 1.12-3.25). No significant differential association was observed for peritumoral lymphocytic reaction, Crohn-like lymphoid reaction, or T-cell densities. In conclusion, the association of F. nucleatum with immune response to colorectal carcinoma differs by tumor MSI status, suggesting that F. nucleatum and MSI status interact to affect antitumor immune reactions. Cancer Immunol Res; 6(11); 1327-36.",
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T1 - Fusobacterium nucleatum in Colorectal Cancer Relates to Immune Response Differentially by Tumor Microsatellite Instability Status

AU - Hamada, Tsuyoshi

AU - Zhang, Xuehong

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AU - Bullman, Susan

AU - Sukawa, Yasutaka

AU - Nowak, Jonathan A.

AU - Kosumi, Keisuke

AU - Masugi, Yohei

AU - Twombly, Tyler S.

AU - Cao, Yin

AU - Song, Mingyang

AU - Liu, Li

AU - da Silva, Annacarolina

AU - Shi, Yan

AU - Gu, Mancang

AU - Li, Wanwan

AU - Koh, Hideo

AU - Nosho, Katsuhiko

AU - Inamura, Kentaro

AU - Keum, Na Na

AU - Wu, Kana

AU - Meyerhardt, Jeffrey A.

AU - Kostic, Aleksandar D.

AU - Huttenhower, Curtis

AU - Garrett, Wendy S.

AU - Meyerson, Matthew

AU - Giovannucci, Edward L.

AU - Chan, Andrew T.

AU - Fuchs, Charles S.

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AU - Giannakis, Marios

AU - Ogino, Shuji

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N2 - The presence of Fusobacterium nucleatum (F. nucleatum) in colorectal carcinoma tissue has been associated with microsatellite instability (MSI), lower-level T-cell infiltrates, and poor clinical outcomes. Considering differences in the tumor-immune microenvironment between MSI-high and non-MSI-high carcinomas, we hypothesized that the association of F. nucleatum with immune response might differ by tumor MSI status. Using samples from 1,041 rectal and colon cancer patients within the Nurses' Health Study and Health Professionals Follow-up Study, we measured F. nucleatum DNA in tumor tissue by a quantitative polymerase chain reaction assay. Multivariable logistic regression models were used to examine the association between F. nucleatum status and histopathologic lymphocytic reactions or density of CD3+ cells, CD8+ cells, CD45RO (PTPRC)+ cells, or FOXP3+ cells in strata of tumor MSI status. We adjusted for potential confounders, including CpG island methylator phenotype; LINE-1 methylation; and KRAS, BRAF, and PIK3CA mutations. The association of F. nucleatum with tumor-infiltrating lymphocytes (TIL) and intratumoral periglandular reaction differed by tumor MSI status (Pinteraction = 0.002). The presence of F. nucleatum was negatively associated with TIL in MSI-high tumors [multivariable odds ratio (OR), 0.45; 95% confidence interval (CI), 0.22-0.92], but positively associated with TIL in non-MSI-high tumors (multivariable OR 1.91; 95% CI, 1.12-3.25). No significant differential association was observed for peritumoral lymphocytic reaction, Crohn-like lymphoid reaction, or T-cell densities. In conclusion, the association of F. nucleatum with immune response to colorectal carcinoma differs by tumor MSI status, suggesting that F. nucleatum and MSI status interact to affect antitumor immune reactions. Cancer Immunol Res; 6(11); 1327-36.

AB - The presence of Fusobacterium nucleatum (F. nucleatum) in colorectal carcinoma tissue has been associated with microsatellite instability (MSI), lower-level T-cell infiltrates, and poor clinical outcomes. Considering differences in the tumor-immune microenvironment between MSI-high and non-MSI-high carcinomas, we hypothesized that the association of F. nucleatum with immune response might differ by tumor MSI status. Using samples from 1,041 rectal and colon cancer patients within the Nurses' Health Study and Health Professionals Follow-up Study, we measured F. nucleatum DNA in tumor tissue by a quantitative polymerase chain reaction assay. Multivariable logistic regression models were used to examine the association between F. nucleatum status and histopathologic lymphocytic reactions or density of CD3+ cells, CD8+ cells, CD45RO (PTPRC)+ cells, or FOXP3+ cells in strata of tumor MSI status. We adjusted for potential confounders, including CpG island methylator phenotype; LINE-1 methylation; and KRAS, BRAF, and PIK3CA mutations. The association of F. nucleatum with tumor-infiltrating lymphocytes (TIL) and intratumoral periglandular reaction differed by tumor MSI status (Pinteraction = 0.002). The presence of F. nucleatum was negatively associated with TIL in MSI-high tumors [multivariable odds ratio (OR), 0.45; 95% confidence interval (CI), 0.22-0.92], but positively associated with TIL in non-MSI-high tumors (multivariable OR 1.91; 95% CI, 1.12-3.25). No significant differential association was observed for peritumoral lymphocytic reaction, Crohn-like lymphoid reaction, or T-cell densities. In conclusion, the association of F. nucleatum with immune response to colorectal carcinoma differs by tumor MSI status, suggesting that F. nucleatum and MSI status interact to affect antitumor immune reactions. Cancer Immunol Res; 6(11); 1327-36.

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