Fusogenic liposomes prepared with polyethyleneglycol-lipid

J. Sunamoto, K. Tanaka, T. Saito, K. Akiyoshi, Toshinori Sato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Liposomes have been widely accepted in biotechnology and medicine as a vehicle for the delivery into cells of various substances, eg. DNA, RNA, enzymes, or drugs. In order for biologically unstable compounds to be internalized into cells, fusion is favored because the liposome-loading compounds are directly delivered into the cytosol and able to avoid destruction by lysosomal enzymes. From this point of view, we have developed a fusogenic liposome, which contains an artificial lipid, polyethyleneglycol-lipid (PEG-lipid) or polyethyleneglycol-substituted cholesterol (PEG-chol). The fusogenicity of these functionalized liposomes to carrot protoplasts and human lymphocytes was investigated. Egg PC liposomes with 23 Mole% PEG-lipid (n = 11) were the most effective in fusing with carrot protoplasts. The fusogenicity of PEG-chol containing liposomes was significantly less. The difference in the fusogenicity between the two liposomes is ascribed to the difference in hydration capacity and fluidity of liposomal membranes. In the case of human lymphocytes, fusion was observed only at 4.0 °C, and endocytosis occurred at 37°C.

Original languageEnglish
Title of host publicationPolymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Editors Anon
PublisherPubl by ACS
Pages398
Number of pages1
Volume64
Publication statusPublished - 1991
Externally publishedYes
EventProceedings of the American Chemical Society, Spring Meeting - Atlanta, GA, USA
Duration: 1991 Apr 151991 Apr 19

Other

OtherProceedings of the American Chemical Society, Spring Meeting
CityAtlanta, GA, USA
Period91/4/1591/4/19

Fingerprint

Liposomes
Lipids
Lymphocytes
Cholesterol
Fusion reactions
Enzymes
Fluidity
Biotechnology
RNA
Hydration
Medicine
DNA
Membranes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Polymers and Plastics

Cite this

Sunamoto, J., Tanaka, K., Saito, T., Akiyoshi, K., & Sato, T. (1991). Fusogenic liposomes prepared with polyethyleneglycol-lipid. In Anon (Ed.), Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering (Vol. 64, pp. 398). Publ by ACS.

Fusogenic liposomes prepared with polyethyleneglycol-lipid. / Sunamoto, J.; Tanaka, K.; Saito, T.; Akiyoshi, K.; Sato, Toshinori.

Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. ed. / Anon. Vol. 64 Publ by ACS, 1991. p. 398.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sunamoto, J, Tanaka, K, Saito, T, Akiyoshi, K & Sato, T 1991, Fusogenic liposomes prepared with polyethyleneglycol-lipid. in Anon (ed.), Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. vol. 64, Publ by ACS, pp. 398, Proceedings of the American Chemical Society, Spring Meeting, Atlanta, GA, USA, 91/4/15.
Sunamoto J, Tanaka K, Saito T, Akiyoshi K, Sato T. Fusogenic liposomes prepared with polyethyleneglycol-lipid. In Anon, editor, Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. Vol. 64. Publ by ACS. 1991. p. 398
Sunamoto, J. ; Tanaka, K. ; Saito, T. ; Akiyoshi, K. ; Sato, Toshinori. / Fusogenic liposomes prepared with polyethyleneglycol-lipid. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. editor / Anon. Vol. 64 Publ by ACS, 1991. pp. 398
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