Fusogenic liposomes prepared with polyethyleneglycol-lipid

J. Sunamoto, K. Tanaka, T. Saito, K. Akiyoshi, T. Sato

Research output: Contribution to journalConference articlepeer-review

Abstract

Liposomes have been widely accepted in biotechnology and medicine as a vehicle for the delivery into cells of various substances, eg. DNA, RNA, enzymes, or drugs. In order for biologically unstable compounds to be internalized into cells, fusion is favored because the liposome-loading compounds are directly delivered into the cytosol and able to avoid destruction by lysosomal enzymes. From this point of view, we have developed a fusogenic liposome, which contains an artificial lipid, polyethyleneglycol-lipid (PEG-lipid) or polyethyleneglycol-substituted cholesterol (PEG-chol). The fusogenicity of these functionalized liposomes to carrot protoplasts and human lymphocytes was investigated. Egg PC liposomes with 23 Mole% PEG-lipid (n = 11) were the most effective in fusing with carrot protoplasts. The fusogenicity of PEG-chol containing liposomes was significantly less. The difference in the fusogenicity between the two liposomes is ascribed to the difference in hydration capacity and fluidity of liposomal membranes. In the case of human lymphocytes, fusion was observed only at 4.0 °C, and endocytosis occurred at 37°C.

Original languageEnglish
Number of pages1
JournalPolymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Volume64
Publication statusPublished - 1991 Jan 1
Externally publishedYes
EventProceedings of the American Chemical Society, Spring Meeting - Atlanta, GA, USA
Duration: 1991 Apr 151991 Apr 19

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Polymers and Plastics

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