G-CSF augments small vessel and cell density in canine myocardial infarciton

Takashi Yagi, Keiichi Fukuda, Jun Fujita, Jin Endo, Yasuyo Hisaka, Yoshiyuki Suzuki, Masahiko Tamura, Satoshi Ogawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We recently reported that granulocyte-colony stimulating factor (G-CSF) prevented cardiac remodeling by mobilization and differentiation of bone marrow-derived cells in murine experimental myocardial infarction (MI). Little is known, however, whether these findings can be reproduced in large animals. The aim of this study is to investigate the effect of G-CSF after MI in canine model. MI was generated in twenty-six beagle dogs by ligation of left anterior descending artery. They were divided into two groups: G-CSF group which received subcutaneous injection of G-CSF (10 μg/kg/day) for 10 days, and the control group with saline injection. After six weeks, they were subjected to echocardiography and catheterization to measure hemodynamic parameters, and histological analysis was performed. No dogs died during the period. No hemodynamic changes were observed between these two groups probably due to the smaller size of the MI than we expected. We found significant increase in wall thickness and higher cell density in G-CSF group. Immunohistochemical staining against ?-smooth muscle actin and CD31 revealed increased vessel density mainly in the epicardium in G-CSF group. The number of survived cardiomyocytes in G-CSF group was slightly greater than that in the control group, although it was not statistically significant. These findings suggested G-CSF prevented cardiac remodeling in canine model not by increasing the cardiomyocytes but by increasing the vessel density and cell numbers in the infarcted area.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalKeio Journal of Medicine
Volume57
Issue number3
DOIs
Publication statusPublished - 2008 Sep

Fingerprint

Granulocyte Colony-Stimulating Factor
Canidae
Cell Count
Myocardial Infarction
Cardiac Myocytes
Hemodynamics
Dogs
Control Groups
Pericardium
Subcutaneous Injections
Catheterization
Bone Marrow Cells
Ligation
Smooth Muscle
Echocardiography
Actins
Arteries
Staining and Labeling
Injections

Keywords

  • Bone marrow derived cell
  • Cardiac remodeling
  • G-CSF
  • Myocardial infarction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

G-CSF augments small vessel and cell density in canine myocardial infarciton. / Yagi, Takashi; Fukuda, Keiichi; Fujita, Jun; Endo, Jin; Hisaka, Yasuyo; Suzuki, Yoshiyuki; Tamura, Masahiko; Ogawa, Satoshi.

In: Keio Journal of Medicine, Vol. 57, No. 3, 09.2008, p. 139-149.

Research output: Contribution to journalArticle

Yagi, T, Fukuda, K, Fujita, J, Endo, J, Hisaka, Y, Suzuki, Y, Tamura, M & Ogawa, S 2008, 'G-CSF augments small vessel and cell density in canine myocardial infarciton', Keio Journal of Medicine, vol. 57, no. 3, pp. 139-149. https://doi.org/10.2302/kjm.57.139
Yagi, Takashi ; Fukuda, Keiichi ; Fujita, Jun ; Endo, Jin ; Hisaka, Yasuyo ; Suzuki, Yoshiyuki ; Tamura, Masahiko ; Ogawa, Satoshi. / G-CSF augments small vessel and cell density in canine myocardial infarciton. In: Keio Journal of Medicine. 2008 ; Vol. 57, No. 3. pp. 139-149.
@article{818eb430f689446a871af5383c453098,
title = "G-CSF augments small vessel and cell density in canine myocardial infarciton",
abstract = "We recently reported that granulocyte-colony stimulating factor (G-CSF) prevented cardiac remodeling by mobilization and differentiation of bone marrow-derived cells in murine experimental myocardial infarction (MI). Little is known, however, whether these findings can be reproduced in large animals. The aim of this study is to investigate the effect of G-CSF after MI in canine model. MI was generated in twenty-six beagle dogs by ligation of left anterior descending artery. They were divided into two groups: G-CSF group which received subcutaneous injection of G-CSF (10 μg/kg/day) for 10 days, and the control group with saline injection. After six weeks, they were subjected to echocardiography and catheterization to measure hemodynamic parameters, and histological analysis was performed. No dogs died during the period. No hemodynamic changes were observed between these two groups probably due to the smaller size of the MI than we expected. We found significant increase in wall thickness and higher cell density in G-CSF group. Immunohistochemical staining against ?-smooth muscle actin and CD31 revealed increased vessel density mainly in the epicardium in G-CSF group. The number of survived cardiomyocytes in G-CSF group was slightly greater than that in the control group, although it was not statistically significant. These findings suggested G-CSF prevented cardiac remodeling in canine model not by increasing the cardiomyocytes but by increasing the vessel density and cell numbers in the infarcted area.",
keywords = "Bone marrow derived cell, Cardiac remodeling, G-CSF, Myocardial infarction",
author = "Takashi Yagi and Keiichi Fukuda and Jun Fujita and Jin Endo and Yasuyo Hisaka and Yoshiyuki Suzuki and Masahiko Tamura and Satoshi Ogawa",
year = "2008",
month = "9",
doi = "10.2302/kjm.57.139",
language = "English",
volume = "57",
pages = "139--149",
journal = "Keio Journal of Medicine",
issn = "0022-9717",
publisher = "Keio University School of Medicine",
number = "3",

}

TY - JOUR

T1 - G-CSF augments small vessel and cell density in canine myocardial infarciton

AU - Yagi, Takashi

AU - Fukuda, Keiichi

AU - Fujita, Jun

AU - Endo, Jin

AU - Hisaka, Yasuyo

AU - Suzuki, Yoshiyuki

AU - Tamura, Masahiko

AU - Ogawa, Satoshi

PY - 2008/9

Y1 - 2008/9

N2 - We recently reported that granulocyte-colony stimulating factor (G-CSF) prevented cardiac remodeling by mobilization and differentiation of bone marrow-derived cells in murine experimental myocardial infarction (MI). Little is known, however, whether these findings can be reproduced in large animals. The aim of this study is to investigate the effect of G-CSF after MI in canine model. MI was generated in twenty-six beagle dogs by ligation of left anterior descending artery. They were divided into two groups: G-CSF group which received subcutaneous injection of G-CSF (10 μg/kg/day) for 10 days, and the control group with saline injection. After six weeks, they were subjected to echocardiography and catheterization to measure hemodynamic parameters, and histological analysis was performed. No dogs died during the period. No hemodynamic changes were observed between these two groups probably due to the smaller size of the MI than we expected. We found significant increase in wall thickness and higher cell density in G-CSF group. Immunohistochemical staining against ?-smooth muscle actin and CD31 revealed increased vessel density mainly in the epicardium in G-CSF group. The number of survived cardiomyocytes in G-CSF group was slightly greater than that in the control group, although it was not statistically significant. These findings suggested G-CSF prevented cardiac remodeling in canine model not by increasing the cardiomyocytes but by increasing the vessel density and cell numbers in the infarcted area.

AB - We recently reported that granulocyte-colony stimulating factor (G-CSF) prevented cardiac remodeling by mobilization and differentiation of bone marrow-derived cells in murine experimental myocardial infarction (MI). Little is known, however, whether these findings can be reproduced in large animals. The aim of this study is to investigate the effect of G-CSF after MI in canine model. MI was generated in twenty-six beagle dogs by ligation of left anterior descending artery. They were divided into two groups: G-CSF group which received subcutaneous injection of G-CSF (10 μg/kg/day) for 10 days, and the control group with saline injection. After six weeks, they were subjected to echocardiography and catheterization to measure hemodynamic parameters, and histological analysis was performed. No dogs died during the period. No hemodynamic changes were observed between these two groups probably due to the smaller size of the MI than we expected. We found significant increase in wall thickness and higher cell density in G-CSF group. Immunohistochemical staining against ?-smooth muscle actin and CD31 revealed increased vessel density mainly in the epicardium in G-CSF group. The number of survived cardiomyocytes in G-CSF group was slightly greater than that in the control group, although it was not statistically significant. These findings suggested G-CSF prevented cardiac remodeling in canine model not by increasing the cardiomyocytes but by increasing the vessel density and cell numbers in the infarcted area.

KW - Bone marrow derived cell

KW - Cardiac remodeling

KW - G-CSF

KW - Myocardial infarction

UR - http://www.scopus.com/inward/record.url?scp=56049104002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56049104002&partnerID=8YFLogxK

U2 - 10.2302/kjm.57.139

DO - 10.2302/kjm.57.139

M3 - Article

C2 - 18854666

AN - SCOPUS:56049104002

VL - 57

SP - 139

EP - 149

JO - Keio Journal of Medicine

JF - Keio Journal of Medicine

SN - 0022-9717

IS - 3

ER -