G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae

Keiko Kono, Satoru Nogami, Mitsuhiro Abe, Masafumi Nishizawa, Shinichi Morishita, David Pellman, Yoshikazu Ohya

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Rho1p is an essential small GTPase that plays a key role in the morphogenesis of Saccharomyces cerevisiae. We show here that the activation of Rho1p is regulated by a cyclin-dependent kinase (CDK). Rho1p is activated at the G1/S transition at the incipient-bud sites by the Cln2p (G1 cyclin) and Cdc28p (CDK) complex, in a process mediated by Tus1p, a guanine nucleotide exchange factor for Rho1p. Tus1p interacts physically with Cln2p/Cdc28p and is phosphorylated in a Cln2p/Cdc28p-dependent manner. CDK phosphorylation consensus sites in Tus1p are required for both Cln2p-dependent activation of Rho1p and polarized organization of the actin cytoskeleton. We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition.

Original languageEnglish
Pages (from-to)1763-1771
Number of pages9
JournalMolecular Biology of the Cell
Volume19
Issue number4
DOIs
Publication statusPublished - 2008 Apr

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Rho Guanine Nucleotide Exchange Factors
Monomeric GTP-Binding Proteins
Cyclin-Dependent Kinases
Saccharomyces cerevisiae
Phosphorylation
Cyclin G1
Guanine Nucleotide Exchange Factors
Actin Cytoskeleton
Morphogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae. / Kono, Keiko; Nogami, Satoru; Abe, Mitsuhiro; Nishizawa, Masafumi; Morishita, Shinichi; Pellman, David; Ohya, Yoshikazu.

In: Molecular Biology of the Cell, Vol. 19, No. 4, 04.2008, p. 1763-1771.

Research output: Contribution to journalArticle

Kono, Keiko ; Nogami, Satoru ; Abe, Mitsuhiro ; Nishizawa, Masafumi ; Morishita, Shinichi ; Pellman, David ; Ohya, Yoshikazu. / G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae. In: Molecular Biology of the Cell. 2008 ; Vol. 19, No. 4. pp. 1763-1771.
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AU - Nishizawa, Masafumi

AU - Morishita, Shinichi

AU - Pellman, David

AU - Ohya, Yoshikazu

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AB - Rho1p is an essential small GTPase that plays a key role in the morphogenesis of Saccharomyces cerevisiae. We show here that the activation of Rho1p is regulated by a cyclin-dependent kinase (CDK). Rho1p is activated at the G1/S transition at the incipient-bud sites by the Cln2p (G1 cyclin) and Cdc28p (CDK) complex, in a process mediated by Tus1p, a guanine nucleotide exchange factor for Rho1p. Tus1p interacts physically with Cln2p/Cdc28p and is phosphorylated in a Cln2p/Cdc28p-dependent manner. CDK phosphorylation consensus sites in Tus1p are required for both Cln2p-dependent activation of Rho1p and polarized organization of the actin cytoskeleton. We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition.

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