G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to lowor high-LET radiation

T. Kawata, H. Ito, T. Uno, M. Saito, S. Yamamoto, Y. Furusawa, M. Durante, K. George, H. Wu, F. A. Cucinotta

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Radiation-induced chromosome damage can be measured in interphase using the Premature Chromosome Condensation (PCC) technique. With the introduction of a new PCC technique using the potent phosphatase inhibitor calyculin-A, chromosomes can be condensed within five minutes, and it is now possible to examine the early damage induced by radiation. Using this method, it has been shown that high-LET radiation induces a higher frequency of chromatid breaks and a much higher frequency of isochromatid breaks than low-LET radiation. The kinetics of chromatid break rejoining consists of two exponential components representing a rapid and a slow time constant, which appears to be similar for low- and high-LET radiations. However, after high-LET radiation exposures, the rejoining process for isochromatid breaks influences the repair kinetics of chromatid-type breaks, and this plays an important role in the assessment of chromatid break rejoining in the G2 phase of the cell cycle.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalCytogenetic and Genome Research
Volume104
Issue number1-4
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Linear Energy Transfer
Chromatids
Fibroblasts
Radiation
Chromosomes
G2 Phase
Interphase
Phosphoric Monoester Hydrolases
Cell Cycle

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kawata, T., Ito, H., Uno, T., Saito, M., Yamamoto, S., Furusawa, Y., ... Cucinotta, F. A. (2004). G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to lowor high-LET radiation. Cytogenetic and Genome Research, 104(1-4), 211-215. https://doi.org/10.1159/000077491

G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to lowor high-LET radiation. / Kawata, T.; Ito, H.; Uno, T.; Saito, M.; Yamamoto, S.; Furusawa, Y.; Durante, M.; George, K.; Wu, H.; Cucinotta, F. A.

In: Cytogenetic and Genome Research, Vol. 104, No. 1-4, 2004, p. 211-215.

Research output: Contribution to journalArticle

Kawata, T, Ito, H, Uno, T, Saito, M, Yamamoto, S, Furusawa, Y, Durante, M, George, K, Wu, H & Cucinotta, FA 2004, 'G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to lowor high-LET radiation', Cytogenetic and Genome Research, vol. 104, no. 1-4, pp. 211-215. https://doi.org/10.1159/000077491
Kawata, T. ; Ito, H. ; Uno, T. ; Saito, M. ; Yamamoto, S. ; Furusawa, Y. ; Durante, M. ; George, K. ; Wu, H. ; Cucinotta, F. A. / G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to lowor high-LET radiation. In: Cytogenetic and Genome Research. 2004 ; Vol. 104, No. 1-4. pp. 211-215.
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