G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to lowor high-LET radiation

T. Kawata, H. Ito, T. Uno, M. Saito, S. Yamamoto, Y. Furusawa, M. Durante, K. George, H. Wu, F. A. Cucinotta

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Abstract

Radiation-induced chromosome damage can be measured in interphase using the Premature Chromosome Condensation (PCC) technique. With the introduction of a new PCC technique using the potent phosphatase inhibitor calyculin-A, chromosomes can be condensed within five minutes, and it is now possible to examine the early damage induced by radiation. Using this method, it has been shown that high-LET radiation induces a higher frequency of chromatid breaks and a much higher frequency of isochromatid breaks than low-LET radiation. The kinetics of chromatid break rejoining consists of two exponential components representing a rapid and a slow time constant, which appears to be similar for low- and high-LET radiations. However, after high-LET radiation exposures, the rejoining process for isochromatid breaks influences the repair kinetics of chromatid-type breaks, and this plays an important role in the assessment of chromatid break rejoining in the G2 phase of the cell cycle.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalCytogenetic and Genome Research
Volume104
Issue number1-4
DOIs
Publication statusPublished - 2004 Jun 15

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Kawata, T., Ito, H., Uno, T., Saito, M., Yamamoto, S., Furusawa, Y., Durante, M., George, K., Wu, H., & Cucinotta, F. A. (2004). G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to lowor high-LET radiation. Cytogenetic and Genome Research, 104(1-4), 211-215. https://doi.org/10.1159/000077491