GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis

Sophie Deléhouzée, Tatsufumi Yoshikawa, Chika Sawa, Jun Ichi Sawada, Takumi Ito, Masashi Omori, Tadashi Wada, Yuki Yamaguchi, Yasuaki Kabe, Hiroshi Handa

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Muscle cell differentiation, or myogenesis, is a well-characterized process and involves the expression of specific sets of genes in an orderly manner. A prerequisite for myogenesis is the exit from the cell cycle, which is associated with the up-regulation of the tumor suppressor Rb. In this study, we set to investigate the regulatory mechanism of the Rb promoter that allows adequate up-regulation in differentiating myoblasts.We report that Rb expression is regulated by the transcription factors GABP, HCF-1 and YY1. Before induction of differentiation, Rb is expressed at a low level and GABP and YY1 are both present on the promoter.YY1, which exerts an inhibitory effect on Rb expression, is removed from the promoter as cells advance through myogenesis and translocates from the nucleus to the cytoplasm. On the other hand, upon induction of differentiation, the GABP cofactor HCF-1 is recruited to and coactivates the promoter with GABP. RNAi-mediated knock-down of HCF-1 results in inhibition of Rb up-regulation as well as myotube formation. These results indicate that the Rb promoter is subject to regulation by positive and negative factors and that this intricate activation mechanism is critical to allow the accurate Rb gene up-regulation observed during myogenesis.

Original languageEnglish
Pages (from-to)717-731
Number of pages15
JournalGenes to Cells
Volume10
Issue number7
DOIs
Publication statusPublished - 2005 Jul
Externally publishedYes

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Retinoblastoma Genes
Muscle Development
Up-Regulation
Gene Expression
GA-Binding Protein Transcription Factor
Myoblasts
Skeletal Muscle Fibers
RNA Interference
Muscle Cells
Cell Differentiation
Cell Cycle
Cytoplasm
Genes
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Deléhouzée, S., Yoshikawa, T., Sawa, C., Sawada, J. I., Ito, T., Omori, M., ... Handa, H. (2005). GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis. Genes to Cells, 10(7), 717-731. https://doi.org/10.1111/j.1365-2443.2005.00873.x

GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis. / Deléhouzée, Sophie; Yoshikawa, Tatsufumi; Sawa, Chika; Sawada, Jun Ichi; Ito, Takumi; Omori, Masashi; Wada, Tadashi; Yamaguchi, Yuki; Kabe, Yasuaki; Handa, Hiroshi.

In: Genes to Cells, Vol. 10, No. 7, 07.2005, p. 717-731.

Research output: Contribution to journalArticle

Deléhouzée, S, Yoshikawa, T, Sawa, C, Sawada, JI, Ito, T, Omori, M, Wada, T, Yamaguchi, Y, Kabe, Y & Handa, H 2005, 'GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis', Genes to Cells, vol. 10, no. 7, pp. 717-731. https://doi.org/10.1111/j.1365-2443.2005.00873.x
Deléhouzée S, Yoshikawa T, Sawa C, Sawada JI, Ito T, Omori M et al. GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis. Genes to Cells. 2005 Jul;10(7):717-731. https://doi.org/10.1111/j.1365-2443.2005.00873.x
Deléhouzée, Sophie ; Yoshikawa, Tatsufumi ; Sawa, Chika ; Sawada, Jun Ichi ; Ito, Takumi ; Omori, Masashi ; Wada, Tadashi ; Yamaguchi, Yuki ; Kabe, Yasuaki ; Handa, Hiroshi. / GABP, HCF-1 and YY1 are involved in Rb gene expression during myogenesis. In: Genes to Cells. 2005 ; Vol. 10, No. 7. pp. 717-731.
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