GAD-reactive T cells were mainly detected in autoimmune-related type 1 diabetic patients with HLA DR9

Arata Itoh, Akira Shimada, Keiichi Kodama, Jiro Morimoto, Ryuji Suzuki, Yoichi Oikawa, Junichiro Irie, Yoshinori Nakagawa, Toshikatsu Shigihara, Yasuhiko Kanazawa, Yoshiaki Okubo, Yoshiko Motohashi, Taro Maruyama, Takao Saruta

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Type 1 diabetes mellitus (T1DM) is considered to be a T cell-mediated disease, and many reports suggest that some HLA types, especially HLA DR4 and DR9, convey susceptibility to T1DM in Japanese. We investigated the association between T cell reactivity against GAD and HLA types in "islet-associated autoantibody-positive" T1DM in Japanese. Blood samples were obtained from 36 "autoantibody-positive" type 1 diabetic patients with HLA DR4 or DR9 and 23 type 2 diabetic patients with HLA DR4 or DR9 as controls. They were divided into three groups, DR4/9, DR4/X, and DR9/X groups. In each HLA type group, GAD-reactive IFN-γ-preducing CD4+ cells were assessed by means of intracellular cytokine staining for flow cytometry. Type 1 diabetic patients with HLA DR9/X had significantly higher numbers of GAD-reactive IFN-γ-producing CD4+ cells as compared to type 1 diabetic patients with DR4/X or DR4/9 (P < 0.05) and all type 2 diabetic patients. There was no significant difference in the number of GAD-reactive IFN-γ-producing CD4+ cells between type 1 diabetic and type 2 diabetic patients belonging to the DR4/X and DR4/9 groups. There was an association between T cell reactivity against GAD and HLA DR9 in Japanese type 1 diabetes.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalAnnals of the New York Academy of Sciences
Volume1037
DOIs
Publication statusPublished - 2004 Jan 1

Keywords

  • Cytokine
  • GAD
  • HLA
  • T cell
  • Type 1 diabetes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Itoh, A., Shimada, A., Kodama, K., Morimoto, J., Suzuki, R., Oikawa, Y., Irie, J., Nakagawa, Y., Shigihara, T., Kanazawa, Y., Okubo, Y., Motohashi, Y., Maruyama, T., & Saruta, T. (2004). GAD-reactive T cells were mainly detected in autoimmune-related type 1 diabetic patients with HLA DR9. Annals of the New York Academy of Sciences, 1037, 33-40. https://doi.org/10.1196/annals.1337.006