TY - JOUR
T1 - Gadolinium-Complexed Carboxylated Nanodiamond Particles for Magnetic Resonance Imaging of the Lymphatic System
AU - Yano, Kosaku
AU - Matsumoto, Tomohiro
AU - Okamoto, Yutaka
AU - Bito, Kenta
AU - Kurokawa, Naruki
AU - Hasebe, Terumitsu
AU - Hotta, Atsushi
N1 - Funding Information:
This work was supported in part by Grant-in-Aid for Scientific Research (A) (No. 19H00831 to A.H.) and in part by Grant-in-Aid for Challenging Exploratory Research (No. 19K22067 to A.H.), both from the Japan Society for the Promotion of Science (JSPS: “KAKENHI”). The work was also supported in part by a Grant-in-Aid for Scientific Research (C) (No. 18K07647 to T.M.) from JSPS and in part by an AMED Grant (No. JP18hm0102059s0201 to T.H. and A.H.), Japan. MRI imaging was performed at Tokai University Hachioji Hospital, Japan.
Publisher Copyright:
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PY - 2021/2/26
Y1 - 2021/2/26
N2 - MRI contrast agents with a size of 3-10 nm are considered to be an effective pathway for selective MR lymphatic imaging. In our previous study, we fabricated nanosized MRI contrast agents (Gd-DTPA-ND) with the condensation of nanodiamond (ND) particles and gadolinium chelates (Gd-DTPA). However, these Gd-DTPA-ND particles formed microscale aggregates in distilled water, exceeding the size required for selective lymphatic MR imaging. In this study, carboxylated nanodiamond (CND) particles were utilized as alternative platforms for condensation with gadolinium complexes. The carboxyl groups introduced by oxidation were expected to induce hydrophilicity with a negative charge on the ND surface, resulting in the high dispersity of gadolinium-complexed CND particles. The fabricated Gd-DTPA-CND particles possessed a hydrodynamic diameter of around 4-5 nm without any signs of aggregation in distilled water and human serum. Furthermore, the T1-weighted image of Gd-DTPA-CND particles in distilled water and human serum presented strong contrast, indicating the excellent MRI visibility of the particles. Therefore, the Gd-DTPA-CND particles can become practical MRI contrast agents for the achievement of selective MR imaging of the lymphatic system.
AB - MRI contrast agents with a size of 3-10 nm are considered to be an effective pathway for selective MR lymphatic imaging. In our previous study, we fabricated nanosized MRI contrast agents (Gd-DTPA-ND) with the condensation of nanodiamond (ND) particles and gadolinium chelates (Gd-DTPA). However, these Gd-DTPA-ND particles formed microscale aggregates in distilled water, exceeding the size required for selective lymphatic MR imaging. In this study, carboxylated nanodiamond (CND) particles were utilized as alternative platforms for condensation with gadolinium complexes. The carboxyl groups introduced by oxidation were expected to induce hydrophilicity with a negative charge on the ND surface, resulting in the high dispersity of gadolinium-complexed CND particles. The fabricated Gd-DTPA-CND particles possessed a hydrodynamic diameter of around 4-5 nm without any signs of aggregation in distilled water and human serum. Furthermore, the T1-weighted image of Gd-DTPA-CND particles in distilled water and human serum presented strong contrast, indicating the excellent MRI visibility of the particles. Therefore, the Gd-DTPA-CND particles can become practical MRI contrast agents for the achievement of selective MR imaging of the lymphatic system.
KW - diamond
KW - lymphatic system
KW - magnetic resonance imaging
KW - nanoparticles
KW - size distribution
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U2 - 10.1021/acsanm.0c03165
DO - 10.1021/acsanm.0c03165
M3 - Article
AN - SCOPUS:85101000817
VL - 4
SP - 1702
EP - 1711
JO - ACS Applied Nano Materials
JF - ACS Applied Nano Materials
SN - 2574-0970
IS - 2
ER -