GAL4/UAS-WGA system as a powerful tool for tracing Drosophila transsynaptic neural pathways

Katsuhiko Tabuchi, Kazunobu Sawamoto, Emiko Suzuki, Koichi Ozaki, Masaki Sone, Chihiro Hama, Takako Tanifuji-Morimoto, Yoshihiro Yuasa, Yoshihiro Yoshihara, Akinao Nose, Hideyuki Okano

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Visualization of specific transsynaptic neural pathways is an indispensable technique for understanding the relationship between structure and function in the nervous system. Here, we demonstrate the application of the wheat germ agglutinin (WGA) transgene technique for tracing transsynaptic neural pathways in Drosophila. The intracellular localization of WGA was examined by immunoelectron microscopy. WGA signals were detected in granule- like structures in both the outer photoreceptor cells expressing WGA and the second-order laminar neurons. Misexpression of tetanus toxin (TNT), which inactivates N-synaptobrevin, in the outer photoreceptor cells resulted in the elimination of on/off transients in electroretinogram (ERG) recordings and in a great reduction in WGA transfer into laminar neurons, suggesting that anterograde WGA transsynaptic transfer is dependent mainly on synaptic transmission. Retrograde WGA transfer was also detected upon its forced expression in muscle cells. WGA primarily expressed in muscle cells was taken up by motoneuron axons and transported to their cell bodies in the ventral nerve cord, suggesting that WGA can trace motoneuronal pathways in combination with the muscle-specific GAL4 driver. Thus, the GAL4/UAS-WGA system should facilitate the dissection of the Drosophila neural circuit formation and/or synaptic activity in various regions and at various developmental stages.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalJournal of Neuroscience Research
Volume59
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Fingerprint

Neural Pathways
Wheat Germ Agglutinins
Drosophila
Photoreceptor Cells
Muscle Cells
R-SNARE Proteins
Tetanus Toxin
Neurons
Immunoelectron Microscopy
Motor Neurons
Transgenes
Synaptic Transmission
Nervous System
Axons
Dissection

Keywords

  • Drosophila
  • Optic lobe
  • Synaptic transmission
  • Transsynaptic tracer
  • WGA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GAL4/UAS-WGA system as a powerful tool for tracing Drosophila transsynaptic neural pathways. / Tabuchi, Katsuhiko; Sawamoto, Kazunobu; Suzuki, Emiko; Ozaki, Koichi; Sone, Masaki; Hama, Chihiro; Tanifuji-Morimoto, Takako; Yuasa, Yoshihiro; Yoshihara, Yoshihiro; Nose, Akinao; Okano, Hideyuki.

In: Journal of Neuroscience Research, Vol. 59, No. 1, 01.01.2000, p. 94-99.

Research output: Contribution to journalArticle

Tabuchi, K, Sawamoto, K, Suzuki, E, Ozaki, K, Sone, M, Hama, C, Tanifuji-Morimoto, T, Yuasa, Y, Yoshihara, Y, Nose, A & Okano, H 2000, 'GAL4/UAS-WGA system as a powerful tool for tracing Drosophila transsynaptic neural pathways', Journal of Neuroscience Research, vol. 59, no. 1, pp. 94-99. https://doi.org/10.1002/(SICI)1097-4547(20000101)59:1<94::AID-JNR11>3.0.CO;2-Q
Tabuchi, Katsuhiko ; Sawamoto, Kazunobu ; Suzuki, Emiko ; Ozaki, Koichi ; Sone, Masaki ; Hama, Chihiro ; Tanifuji-Morimoto, Takako ; Yuasa, Yoshihiro ; Yoshihara, Yoshihiro ; Nose, Akinao ; Okano, Hideyuki. / GAL4/UAS-WGA system as a powerful tool for tracing Drosophila transsynaptic neural pathways. In: Journal of Neuroscience Research. 2000 ; Vol. 59, No. 1. pp. 94-99.
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