Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus

Yoichi Imaizumi, Masanori Sakaguchi, Tsuyoshi Morishita, Mamoru Ito, Françoise Poirier, Kazunobu Sawamoto, Hideyuki Okano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background. In the adult mammalian brain, neural stem cells (NSCs) proliferate in the dentate gyrus (DG) of the hippocampus and generate new neurons throughout life. A multimodal protein, Galectin-1, is expressed in neural progenitor cells (NPCs) and implicated in the proliferation of the NPCs in the DG. However, little is known about its detailed expression profile in the NPCs and functions in adult neurogenesis in the DG. Results. Our immunohistochemical and morphological analysis showed that Galectin-1 was expressed in the type 1 and 2a cells, which are putative NSCs, in the subgranular zone (SGZ) of the adult mouse DG. To study Galectin-1's function in adult hippocampal neurogenesis, we made galectin-1 knock-out mice on the C57BL6 background and characterized the effects on neurogenesis. In the SGZ of the galectin-1 knock-out mice, increased numbers of type 1 cells, DCX-positive immature progenitors, and NeuN-positive newborn neurons were observed. Using triple-labeling immunohistochemistry and morphological analyses, we found that the proliferation of the type-1 cells was increased in the SGZ of the galectin-1 knock-out mice, and we propose that this proliferation is the mechanism for the net increase in the adult neurogenesis in these knock-out mice DG. Conclusions. Galectin-1 is expressed in the neural stem cells and down-regulates neurogenesis in the adult hippocampus.

Original languageEnglish
Article number7
JournalMolecular Brain
Volume4
Issue number1
DOIs
Publication statusPublished - 2011

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Galectin 1
Neurogenesis
Hippocampus
Dentate Gyrus
Stem Cells
Down-Regulation
Knockout Mice
Neural Stem Cells
Neurons
Parahippocampal Gyrus
Brain Stem
Cell Count
Immunohistochemistry
Cell Proliferation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Molecular Biology

Cite this

Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus. / Imaizumi, Yoichi; Sakaguchi, Masanori; Morishita, Tsuyoshi; Ito, Mamoru; Poirier, Françoise; Sawamoto, Kazunobu; Okano, Hideyuki.

In: Molecular Brain, Vol. 4, No. 1, 7, 2011.

Research output: Contribution to journalArticle

Imaizumi, Yoichi ; Sakaguchi, Masanori ; Morishita, Tsuyoshi ; Ito, Mamoru ; Poirier, Françoise ; Sawamoto, Kazunobu ; Okano, Hideyuki. / Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus. In: Molecular Brain. 2011 ; Vol. 4, No. 1.
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AU - Poirier, Françoise

AU - Sawamoto, Kazunobu

AU - Okano, Hideyuki

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