Galectin-1 regulates neurogenesis in the subventricular zone and promotes functional recovery after stroke

Satoru Ishibashi, Toshihiko Kuroiwa, Masanori Sakaguchi, Liyuan Sun, Toshihiko Kadoya, Hideyuki Okano, Hidehiro Mizusawa

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Galectin-1 (Gal-1) has recently been identified as a key molecule that plays important roles in the regulation of neural progenitor cell proliferation in two neurogenic regions: the subventricular zone (SVZ) of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. To test the hypothesis that Gal-1 contributes to adult neurogenesis after focal ischemia, we studied the temporal profile of endogenous Gal-1 expression and the effects of human recombinant Gal-1 on neurogenesis and neurological functions in an experimental focal ischemic model. In the normal brain, Gal-1 expression was observed only in the SVZ. In the ischemic brain, Gal-1 expression was markedly upregulated in the SVZ and the area of selective neuronal death around the infarct in the striatum. The temporal profile of Gal-1 expression was correlated with that of neural progenitor cell proliferation in the SVZ of the ischemic hemisphere. Double-labeling studies revealed that Gal-1 was localized predominantly in both reactive astrocytes and SVZ astrocytes. Administration of Gal-1, which is known to have carbohydrate-binding ability, into the lateral ventricle increased neurogenesis in the ipsilateral SVZ and improved sensorimotor dysfunction after focal ischemia. By contrast, blockade of Gal-1 in the SVZ by the administration of anti-Gal-1 neutralizing antibody strongly inhibited neurogenesis and diminished neurological function. These results suggest that Gal-1 is one of the principal regulators of adult SVZ neurogenesis through its carbohydrate-binding ability and provide evidence that Gal-1 protein has a role in the improvement of sensorimotor function after stroke.

Original languageEnglish
Pages (from-to)302-313
Number of pages12
JournalExperimental Neurology
Volume207
Issue number2
DOIs
Publication statusPublished - 2007 Oct

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Galectin 1
Lateral Ventricles
Neurogenesis
Stroke
Astrocytes
Stem Cells
Ischemia
Carbohydrates
Cell Proliferation
Parahippocampal Gyrus
Dentate Gyrus
Brain
Neutralizing Antibodies

Keywords

  • Behavior, animal
  • Carbohydrate
  • Galectin-1
  • Neural progenitor cell
  • Neurogenesis
  • Regeneration
  • Stroke, experimental
  • Subventricular zone

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Galectin-1 regulates neurogenesis in the subventricular zone and promotes functional recovery after stroke. / Ishibashi, Satoru; Kuroiwa, Toshihiko; Sakaguchi, Masanori; Sun, Liyuan; Kadoya, Toshihiko; Okano, Hideyuki; Mizusawa, Hidehiro.

In: Experimental Neurology, Vol. 207, No. 2, 10.2007, p. 302-313.

Research output: Contribution to journalArticle

Ishibashi, Satoru ; Kuroiwa, Toshihiko ; Sakaguchi, Masanori ; Sun, Liyuan ; Kadoya, Toshihiko ; Okano, Hideyuki ; Mizusawa, Hidehiro. / Galectin-1 regulates neurogenesis in the subventricular zone and promotes functional recovery after stroke. In: Experimental Neurology. 2007 ; Vol. 207, No. 2. pp. 302-313.
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