Galectin-3 is an amplifier of the interleukin-1β-mediated inflammatory response in corneal keratinocytes

Yuichi Uchino, Ashley M. Woodward, Jérôme Mauris, Kristoffer Peterson, Priya Verma, Ulf J. Nilsson, Jaya Rajaiya, Pablo Argüeso

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Interleukin-1β (IL-1β) is a potent mediator of innate immunity commonly up-regulated in a broad spectrum of inflammatory diseases. When bound to its cell surface receptor, IL-1β initiates a signalling cascade that cooperatively induces the expression of canonical IL-1 target genes such as IL-8 and IL-6. Here, we present galectin-3 as a novel regulator of IL-1β responses in corneal keratinocytes. Using the SNAP-tag system and digitonin semi-permeabilization, we show that recombinant exogenous galectin-3 binds to the plasma membrane of keratinocytes and is internalized into cytoplasmic compartments. We find that exogenous galectin-3, but not a dominant negative inhibitor of galectin-3 polymerization lacking the N-terminal domain, exacerbates the response to IL-1β by stimulating the secretion of inflammatory cytokines. The activity of galectin-3 could be reduced by a novel d-galactopyranoside derivative targeting the conserved galactoside-binding site of galectins and did not involve interaction with IL-1 receptor 1 or the induction of endogenous IL-1β. Consistent with these observations, we demonstrate that small interfering RNA-mediated suppression of endogenous galectin-3 expression is sufficient to impair the IL-1β-induced secretion of IL-8 and IL-6 in a p38 mitogen-activated protein kinase-independent manner. Collectively, our findings provide a novel role for galectin-3 as an amplifier of IL-1β responses during epithelial inflammation through an as yet unidentified mechanism.

Original languageEnglish
JournalImmunology
DOIs
Publication statusAccepted/In press - 2018 Jan 1
Externally publishedYes

Fingerprint

Galectin 3
Interleukin-1
Keratinocytes
Interleukin-8
Interleukin-6
Galectins
Galactosides
Digitonin
Interleukin-1 Receptors
Cell Surface Receptors
p38 Mitogen-Activated Protein Kinases
Galactose
Innate Immunity
Polymerization
Small Interfering RNA
Binding Sites
Cell Membrane
Cytokines
Inflammation

Keywords

  • Corneal keratinocyte
  • Galectin-3
  • Innate immunity
  • Interleukin-1β
  • P38 mitogen-activated protein kinase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Uchino, Y., Woodward, A. M., Mauris, J., Peterson, K., Verma, P., Nilsson, U. J., ... Argüeso, P. (Accepted/In press). Galectin-3 is an amplifier of the interleukin-1β-mediated inflammatory response in corneal keratinocytes. Immunology. https://doi.org/10.1111/imm.12899

Galectin-3 is an amplifier of the interleukin-1β-mediated inflammatory response in corneal keratinocytes. / Uchino, Yuichi; Woodward, Ashley M.; Mauris, Jérôme; Peterson, Kristoffer; Verma, Priya; Nilsson, Ulf J.; Rajaiya, Jaya; Argüeso, Pablo.

In: Immunology, 01.01.2018.

Research output: Contribution to journalArticle

Uchino, Yuichi ; Woodward, Ashley M. ; Mauris, Jérôme ; Peterson, Kristoffer ; Verma, Priya ; Nilsson, Ulf J. ; Rajaiya, Jaya ; Argüeso, Pablo. / Galectin-3 is an amplifier of the interleukin-1β-mediated inflammatory response in corneal keratinocytes. In: Immunology. 2018.
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