Ganglioside GD1a negatively regulates matrix metalloproteinase-9 expression in mouse FBJ cell lines at the transcriptional level

Dan Hu, Zhiqiu Man, Pu Wang, Xuan Tan, Xiaodong Wang, Shizuka Takaku, Sumiko Hyuga, Toshinori Sato, XinSheng Yao, Sadako Yamagata, Tatsuya Yamagata

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Mouse FBJ virus-induced osteosarcoma FBJ-S1 cells rich in GD1a are not readily metastatic, whereas FBJ-LL cells with low levels of GD1a are highly metastatic. GD1a was previously shown to suppress metastasis of mouse FBJ cells and to upregulate caveolin-1 and stromal interaction molecule 1 expression. The present study demonstrates that matrix metalloproteinase-9 (MMP-9) expression renders FBJ-LL cells invasive. MMP-9 is inversely regulated by GD1a, based upon four observations: MMP-9 mRNA content was 5 times higher in FBJ-LL cells than FBJ-S1 cells; a GD1a-re-expressing FBJ-LL cell variant produced through β1,4GalNAcT-1 cDNA transfection expressed lower levels of MMP-9; exogenous addition of GD1a to FBJ-LL cells decreased MMP-9 production in a dose- and time-dependent manner; and treatment of GD1a-rich cells with D-PDMP or siRNA targeting St3gal2 decreased GD1a expression, but augmented MMP-9 expression. This is the first report demonstrating that GD1a negatively regulates expression of MMP-9 at the transcriptional level.

Original languageEnglish
Pages (from-to)198-205
Number of pages8
JournalConnective Tissue Research
Volume48
Issue number4
DOIs
Publication statusPublished - 2007 Jul

Fingerprint

Matrix Metalloproteinase 9
Cells
Cell Line
Caveolin 1
GD1a ganglioside
Viruses
Small Interfering RNA
Osteosarcoma
Complementary DNA
Transfection
Messenger RNA
Up-Regulation
Molecules
Neoplasm Metastasis

Keywords

  • D-PDMP
  • Ganglioside
  • GD1a
  • Metastasis
  • MMP-9
  • SiRNA
  • St3gal2

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Nephrology
  • Cell Biology
  • Orthopedics and Sports Medicine

Cite this

Ganglioside GD1a negatively regulates matrix metalloproteinase-9 expression in mouse FBJ cell lines at the transcriptional level. / Hu, Dan; Man, Zhiqiu; Wang, Pu; Tan, Xuan; Wang, Xiaodong; Takaku, Shizuka; Hyuga, Sumiko; Sato, Toshinori; Yao, XinSheng; Yamagata, Sadako; Yamagata, Tatsuya.

In: Connective Tissue Research, Vol. 48, No. 4, 07.2007, p. 198-205.

Research output: Contribution to journalArticle

Hu, D, Man, Z, Wang, P, Tan, X, Wang, X, Takaku, S, Hyuga, S, Sato, T, Yao, X, Yamagata, S & Yamagata, T 2007, 'Ganglioside GD1a negatively regulates matrix metalloproteinase-9 expression in mouse FBJ cell lines at the transcriptional level', Connective Tissue Research, vol. 48, no. 4, pp. 198-205. https://doi.org/10.1080/03008200701458731
Hu, Dan ; Man, Zhiqiu ; Wang, Pu ; Tan, Xuan ; Wang, Xiaodong ; Takaku, Shizuka ; Hyuga, Sumiko ; Sato, Toshinori ; Yao, XinSheng ; Yamagata, Sadako ; Yamagata, Tatsuya. / Ganglioside GD1a negatively regulates matrix metalloproteinase-9 expression in mouse FBJ cell lines at the transcriptional level. In: Connective Tissue Research. 2007 ; Vol. 48, No. 4. pp. 198-205.
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