Ganglioside GD1a regulation of caveolin-1 and Stim1 expression in mouse FBJ cells: Augmented expression of caveolin-1 and Stim1 in cells with increased GD1a content

Li Wang, Shizuka Takaku, Pu Wang, Dan Hu, Sumiko Hyuga, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

GD1a was previously shown responsible for regulating cell motility, cellular adhesiveness to vitronectin, phosphorylation of c-Met and metastatic ability of mouse FBJ osteosarcoma cells. To determine the particular molecules regulated by GD1a, FBJ cells were assessed for tumor-related gene expression by semi-quantitative RT-PCR. Caveolin-1 and stromal interaction molecule 1 (Stim1) expression in FBJ-S1 cells, rich in GD1a, were found to be 6 and 4 times as much, respectively, than in FBJ-LL cells devoid of GD1a. Enhanced production of caveolin-1 in protein was confirmed by Western blotting. A low-metastatic FBJ-LL cell variant, having high GD1a expression through β1-4GalNAcT-1 (GM2/GD2 synthase) cDNA transfection (Hyuga S, et al, Int J Cancer 83: 685-91, 1999), showed enhanced production of caveolin-1 and Stim1 in mRNA and protein, compared to mock-transfectant M5. Incubation of FBJ-M5 cells with exogenous GD1a augmented the expression of caveolin-1 in mRNA and protein and Stim1 in mRNA as well. Treatment of FBJ-S1 with fumonisin B1, an inhibitor of N-acylsphinganine synthesis, for 15 days caused the complete depletion of gangliosides and suppressed the expression of caveolin-1 and Stim1. St3gal5 siRNA transfected cells showed decreased expression of caveolin-1 and Stim1 mRNA, as well as St3gal5 mRNA. These findings clearly indicate ganglioside GD1a to be involved in the regulation of the transformation suppressor genes, caveolin-1 and Stim1. Moreover, treatment with GD1a of mouse melanoma B16 cells and human hepatoma HepG2 cells brought about elevated expression of caveolin-1 and Stim1.

Original languageEnglish
Pages (from-to)303-315
Number of pages13
JournalGlycoconjugate Journal
Volume23
Issue number7-8
DOIs
Publication statusPublished - 2006 Nov

Fingerprint

Caveolin 1
Molecules
(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase
Messenger RNA
Suppressor Genes
Vitronectin
Adhesiveness
Experimental Melanomas
Phosphorylation
Proteins
GD1a ganglioside
Stromal Interaction Molecule 1
Gangliosides
Hep G2 Cells
Osteosarcoma
Gene expression
Small Interfering RNA
Cell Movement
Transfection
Tumors

Keywords

  • Caveolin-1
  • Ganglioside
  • GD1a
  • Metastasis
  • RT-PCR
  • Stim-1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Ganglioside GD1a regulation of caveolin-1 and Stim1 expression in mouse FBJ cells : Augmented expression of caveolin-1 and Stim1 in cells with increased GD1a content. / Wang, Li; Takaku, Shizuka; Wang, Pu; Hu, Dan; Hyuga, Sumiko; Sato, Toshinori; Yamagata, Sadako; Yamagata, Tatsuya.

In: Glycoconjugate Journal, Vol. 23, No. 7-8, 11.2006, p. 303-315.

Research output: Contribution to journalArticle

Wang, Li ; Takaku, Shizuka ; Wang, Pu ; Hu, Dan ; Hyuga, Sumiko ; Sato, Toshinori ; Yamagata, Sadako ; Yamagata, Tatsuya. / Ganglioside GD1a regulation of caveolin-1 and Stim1 expression in mouse FBJ cells : Augmented expression of caveolin-1 and Stim1 in cells with increased GD1a content. In: Glycoconjugate Journal. 2006 ; Vol. 23, No. 7-8. pp. 303-315.
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