Gastrointestinal motility changes and myenteric plexus alterations in spontaneously diabetic biobreeding rats

Ingrid Demedts, Tatsuhiro Masaoka, Sebastien Kindt, Gert De Hertogh, Karel Geboes, Ricard Farré, Pieter Vanden Berghe, Jan Tack

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background/Aims Type 1 diabetes is often accompanied by gastrointestinal motility disturbances. Vagal neuropathy, hyperglycemia, and alterations in the myenteric plexus have been proposed as underlying mechanism. We therefore studied the relationship between vagal function, gastrointestinal motiliy and characteristics of the enteric nervous system in the biobreeding (BB) rat known as model for spontaneous type 1 diabetes. Methods Gastric emptying breath test, small intestinal electromyography, relative risk-interval variability, histology and immunohistochemistry on antral and jejunal segments were performed at 1, 8 and 16 weeks after diabetes onset and on age-matched controls. Results We observed no consistent changes in relative risk-interval variability and gastric emptying rate. There was however, a loss of phases 3 with longer duration of diabetes on small intestinal electromyography. We found a progressive decrease of nitrergic neurons in the myenteric plexus of antrum and jejunum, while numbers of cholinergic nerve were not altered. In addition, a transient inflammatory infiltrate in jejunal wall was found in spontaneous diabetic BB rats at 8 weeks of diabetes. Conclusions In diabetic BB rats, altered small intestinal motor control associated with a loss of myenteric nitric oxide synthase expression occurs, which does not depend on hyperglycemia or vagal dysfunction, and which is preceded by transient intestinal inflammation.

Original languageEnglish
Pages (from-to)161-170
Number of pages10
JournalJournal of Neurogastroenterology and Motility
Volume19
Issue number2
DOIs
Publication statusPublished - 2013 Apr
Externally publishedYes

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Myenteric Plexus
Gastrointestinal Motility
Gastric Emptying
Electromyography
Type 1 Diabetes Mellitus
Hyperglycemia
Nitrergic Neurons
Enteric Nervous System
Breath Tests
Jejunum
Age of Onset
Nitric Oxide Synthase
Cholinergic Agents
Histology
Immunohistochemistry
Inflammation

Keywords

  • Diabetes mellitus
  • Electromyography
  • Enteric nervous system
  • Gastric empyting
  • Inbred BB
  • Rats

ASJC Scopus subject areas

  • Gastroenterology
  • Clinical Neurology

Cite this

Gastrointestinal motility changes and myenteric plexus alterations in spontaneously diabetic biobreeding rats. / Demedts, Ingrid; Masaoka, Tatsuhiro; Kindt, Sebastien; Hertogh, Gert De; Geboes, Karel; Farré, Ricard; Berghe, Pieter Vanden; Tack, Jan.

In: Journal of Neurogastroenterology and Motility, Vol. 19, No. 2, 04.2013, p. 161-170.

Research output: Contribution to journalArticle

Demedts, Ingrid ; Masaoka, Tatsuhiro ; Kindt, Sebastien ; Hertogh, Gert De ; Geboes, Karel ; Farré, Ricard ; Berghe, Pieter Vanden ; Tack, Jan. / Gastrointestinal motility changes and myenteric plexus alterations in spontaneously diabetic biobreeding rats. In: Journal of Neurogastroenterology and Motility. 2013 ; Vol. 19, No. 2. pp. 161-170.
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