Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila

Akihiro Nagao, Kaoru Sato, Kazumichi M. Nishida, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

PIWI-interacting RNAs (piRNAs) are germline-specific small non-coding RNAs that form piRNA-induced silencing complexes (piRISCs) by associating with PIWI proteins, a subclade of the Argonaute proteins predominantly expressed in the germline. piRISCs protect the integrity of the germline genome from invasive transposable DNA elements by silencing them. Multiple piRNA biogenesis factors have been identified in Drosophila. The majority of piRNA factors are localized in the nuage, electron-dense non-membranous cytoplasmic structures located in the perinuclear regions of germ cells. Thus, piRNA biogenesis is thought to occur in the nuage in germ cells. Immunofluorescence analyses of ovaries from piRNA factor mutants have revealed a localization hierarchy of piRNA factors in female nuage. However, whether this hierarchy is female-specific or can also be applied in male gonads remains undetermined. Here, we show by immunostaining of both ovaries and testes from piRNA factor mutants that the molecular hierarchy of piRNA factors shows gender-specificity, especially for Krimper (Krimp), a Tudor-domain-containing protein of unknown function(s): Krimp is dispensable for PIWI protein Aubergine (Aub) nuage localization in ovaries but Krimp and Aub require each other for their proper nuage localization in testes. This suggests that the functional requirement of Krimp in piRNA biogenesis may be different in male and female gonads.

Original languageEnglish
Article numberArticle 55
JournalFrontiers in Genetics
Volume2
Issue numberAUG
DOIs
Publication statusPublished - 2011

Fingerprint

Small Interfering RNA
Drosophila
RNA
Solanum melongena
Ovary
Gonads
Germ Cells
Testis
Argonaute Proteins
Cytoplasmic Structures
Small Untranslated RNA
Proteins
DNA Transposable Elements
Fluorescent Antibody Technique
Genome
Electrons

Keywords

  • Drosophila
  • Germline
  • Nuage
  • piRNA
  • PIWI

ASJC Scopus subject areas

  • Genetics
  • Molecular Medicine
  • Genetics(clinical)

Cite this

Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila. / Nagao, Akihiro; Sato, Kaoru; Nishida, Kazumichi M.; Siomi, Haruhiko; Siomi, Mikiko C.

In: Frontiers in Genetics, Vol. 2, No. AUG, Article 55, 2011.

Research output: Contribution to journalArticle

Nagao, Akihiro ; Sato, Kaoru ; Nishida, Kazumichi M. ; Siomi, Haruhiko ; Siomi, Mikiko C. / Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila. In: Frontiers in Genetics. 2011 ; Vol. 2, No. AUG.
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