Abstract
We cloned two operons for putative RND-type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ(-opmE) and in another operon mexMN. Introduction of the mexPQ-opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ-OpmE and MexMN-OprM are functional multidrug efflux pumps when expressed in P. aeruginosa.
| Original language | English |
|---|---|
| Pages (from-to) | 999-1002 |
| Number of pages | 4 |
| Journal | Microbiology and Immunology |
| Volume | 49 |
| Issue number | 11 |
| Publication status | Published - 2005 |
| Externally published | Yes |
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Keywords
- MexMN
- MexPQ
- Multidrug efflux pumps
- P. aeruginosa
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology (medical)
- Microbiology
Cite this
Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa. / Mima, Takehiko; Sekiya, Hiroshi; Mizushima, Tohru; Kuroda, Teruo; Tsuchiya, Tomofusa.
In: Microbiology and Immunology, Vol. 49, No. 11, 2005, p. 999-1002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa
AU - Mima, Takehiko
AU - Sekiya, Hiroshi
AU - Mizushima, Tohru
AU - Kuroda, Teruo
AU - Tsuchiya, Tomofusa
PY - 2005
Y1 - 2005
N2 - We cloned two operons for putative RND-type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ(-opmE) and in another operon mexMN. Introduction of the mexPQ-opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ-OpmE and MexMN-OprM are functional multidrug efflux pumps when expressed in P. aeruginosa.
AB - We cloned two operons for putative RND-type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ(-opmE) and in another operon mexMN. Introduction of the mexPQ-opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ-OpmE and MexMN-OprM are functional multidrug efflux pumps when expressed in P. aeruginosa.
KW - MexMN
KW - MexPQ
KW - Multidrug efflux pumps
KW - P. aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=28044451986&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=28044451986&partnerID=8YFLogxK
M3 - Article
C2 - 16301811
AN - SCOPUS:28044451986
VL - 49
SP - 999
EP - 1002
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 11
ER -