Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5

Kuniya Abe, Misako Yuzuriha, Michihiko Sugimoto, Minoru Ko, Mgavi Brathwaite, Paul Waeltz, Ramaiah Nagaraja

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mice homozygous for the tw5 allele arrest at gastrulation from defects associated with embryonic ectoderm development. The mutated gene has been genetically closely linked to the H-2K locus in the mouse MHC region, flanked by markers H-2Pb and D17Mit147. Aiming at the positional cloning of the mutated gene, we constructed a BAC contig spanning about 1 Mb of the genomic region. On the basis of our mapping and sequencing analysis of the BACs combined with public genome data, EST database searches, and gene prediction programs, we delimit the 1.06 cM of the tw5 critical region to 750 kb, and infer 36 genes (1/20 kb) encoded in the interval. All of the 33 genes tested were confirmed as expressed in embryonic tissues by RT-PCR analyses, and in many cases by EST expression profiles as well. Thus, this highly gene-rich region is essentially totally transcribed during early development and provides priority candidates to be screened for the tw5 embryonic lesion.

Original languageEnglish
Pages (from-to)265-276
Number of pages12
JournalMammalian Genome
Volume15
Issue number4
DOIs
Publication statusPublished - 2004 Apr
Externally publishedYes

Fingerprint

Ectoderm
Genes
Expressed Sequence Tags
Gastrulation
Embryonic Development
Organism Cloning
Alleles
Genome
Databases
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Abe, K., Yuzuriha, M., Sugimoto, M., Ko, M., Brathwaite, M., Waeltz, P., & Nagaraja, R. (2004). Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5. Mammalian Genome, 15(4), 265-276. https://doi.org/10.1007/s00335-003-2329-1

Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5. / Abe, Kuniya; Yuzuriha, Misako; Sugimoto, Michihiko; Ko, Minoru; Brathwaite, Mgavi; Waeltz, Paul; Nagaraja, Ramaiah.

In: Mammalian Genome, Vol. 15, No. 4, 04.2004, p. 265-276.

Research output: Contribution to journalArticle

Abe, K, Yuzuriha, M, Sugimoto, M, Ko, M, Brathwaite, M, Waeltz, P & Nagaraja, R 2004, 'Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5', Mammalian Genome, vol. 15, no. 4, pp. 265-276. https://doi.org/10.1007/s00335-003-2329-1
Abe, Kuniya ; Yuzuriha, Misako ; Sugimoto, Michihiko ; Ko, Minoru ; Brathwaite, Mgavi ; Waeltz, Paul ; Nagaraja, Ramaiah. / Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5. In: Mammalian Genome. 2004 ; Vol. 15, No. 4. pp. 265-276.
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