Gene expression analysis of zebrafish heart regeneration

Ching Ling Lien, Michael Schebesta, Shinji Makino, Gerhard J. Weber, Mark T. Keating

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193 Citations (Scopus)

Abstract

Mammalian hearts cannot regenerate. In contrast, zebrafish hearts regenerate even when up to 20% of the ventricle is amputated. The mechanism of zebrafish heart regeneration is not understood. To systematically characterize this process at the molecular level, we generated transcriptional profiles of zebrafish cardiac regeneration by microarray analyses. Distinct gene clusters were identified based on temporal expression patterns. Genes coding for wound response/inflammatory factors, secreted molecules, and matrix metalloproteinases are expressed in regenerating heart in sequential patterns. Comparisons of gene expression profiles between heart and fin regeneration revealed a set of regeneration core molecules as well as tissue-specific factors. The expression patterns of several secreted molecules around the wound suggest that they play important roles in heart regeneration. We found that both platelet-derived growth factor-a and -b (pdgf-a and pdgf-b) are upregulated in regenerating zebrafish hearts. PDGF-B homodimers induce DNA synthesis in adult zebrafish cardiomyocytes. In addition, we demonstrate that a chemical inhibitor of PDGF receptor decreases DNA synthesis of cardiomyocytes both in vitro and in vivo during regeneration. Our data indicate that zebrafish heart regeneration is associated with sequentially upregulated wound healing genes and growth factors and suggest that PDGF signaling is required.

Original languageEnglish
Pages (from-to)1386-1396
Number of pages11
JournalPLoS biology
Volume4
Issue number8
DOIs
Publication statusPublished - 2006 Aug 23
Externally publishedYes

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lien, C. L., Schebesta, M., Makino, S., Weber, G. J., & Keating, M. T. (2006). Gene expression analysis of zebrafish heart regeneration. PLoS biology, 4(8), 1386-1396. https://doi.org/10.1371/journal.pbio.0040260