Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary

Seema Sharma, Souichi Oomizu, Tomoshi Kakeya, Toru Masui, Sakae Takeuchi, Sumio Takahashi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Transforming growth factor-α (TGF-α, a member of the epidermal growth factor (EGF) family, is produced within the mouse anterior pituitaries. However, the cell types of TGF-α-expressing cells and the physiological roles of TGF-α within mouse pituitary glands remain unclear. The aim of the present study was to localize TGF-α mRNA-expressing cells, and to clarify the involvement of TGF-α in estrogen-induced DNA replication in mouse anterior pituitary cells. Northern blot analysis demonstrated TGF-α mRNA expression in adult male and female mouse anterior pituitaries. In situ hybridization analysis of the pituitaries in these mice showed that TGF-α mRNA-expressing cells in the anterior pituitary are round, oval, and medium-sized. TGF-α mRNA was colocalized in most of the growth hormone (GH) mRNA-expressing cells, while only some of the prolactin (PRL) mRNA-expressing cells. DNA replication in the anterior pituitary cells was detected by monitoring the cellular uptake of a thymidine analogue, bromodeoxyuridine (BrdU) in a primary serum-free culture system. Estradiol-17β (E2) and TGF-α treatment increased the number of BrdU-labelled mammotrophs, indicating that E2 and TGF-α treatment stimulates the DNA replication in mammotrophs. Immunoneutralization of TGF-α with anti-TGF-α-antibodies nullified the E2-induced increase in DNA replication. RT-PCR analysis of TGF-α mRNA expression in ovariectomized female mice revealed that E2 increases TGF-α mRNA levels. These results indicate that the TGF-α produced primarily in the somatotrophs mediates the stimulatory effects of estrogen on the DNA replication of pituitary cells in a paracrine or autocrine manner.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalZoological Science
Volume20
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1
Externally publishedYes

Fingerprint

transforming growth factors
anterior pituitary
gene expression
DNA replication
mice
cells
estrogens
pituitary gland
epidermal growth factor
prolactin
thymidine
Northern blotting
somatotropin
in situ hybridization
estradiol
reverse transcriptase polymerase chain reaction
uptake mechanisms
antibodies
monitoring

Keywords

  • Estrogen
  • Mammotroph
  • Pituitary
  • Somatotroph
  • Transforming growth factor-α (TGF-α)

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Sharma, S., Oomizu, S., Kakeya, T., Masui, T., Takeuchi, S., & Takahashi, S. (2003). Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary. Zoological Science, 20(1), 83-89. https://doi.org/10.2108/zsj.20.83

Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary. / Sharma, Seema; Oomizu, Souichi; Kakeya, Tomoshi; Masui, Toru; Takeuchi, Sakae; Takahashi, Sumio.

In: Zoological Science, Vol. 20, No. 1, 01.01.2003, p. 83-89.

Research output: Contribution to journalArticle

Sharma, S, Oomizu, S, Kakeya, T, Masui, T, Takeuchi, S & Takahashi, S 2003, 'Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary', Zoological Science, vol. 20, no. 1, pp. 83-89. https://doi.org/10.2108/zsj.20.83
Sharma, Seema ; Oomizu, Souichi ; Kakeya, Tomoshi ; Masui, Toru ; Takeuchi, Sakae ; Takahashi, Sumio. / Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary. In: Zoological Science. 2003 ; Vol. 20, No. 1. pp. 83-89.
@article{1be143db0621487792896e37b52d9c14,
title = "Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary",
abstract = "Transforming growth factor-α (TGF-α, a member of the epidermal growth factor (EGF) family, is produced within the mouse anterior pituitaries. However, the cell types of TGF-α-expressing cells and the physiological roles of TGF-α within mouse pituitary glands remain unclear. The aim of the present study was to localize TGF-α mRNA-expressing cells, and to clarify the involvement of TGF-α in estrogen-induced DNA replication in mouse anterior pituitary cells. Northern blot analysis demonstrated TGF-α mRNA expression in adult male and female mouse anterior pituitaries. In situ hybridization analysis of the pituitaries in these mice showed that TGF-α mRNA-expressing cells in the anterior pituitary are round, oval, and medium-sized. TGF-α mRNA was colocalized in most of the growth hormone (GH) mRNA-expressing cells, while only some of the prolactin (PRL) mRNA-expressing cells. DNA replication in the anterior pituitary cells was detected by monitoring the cellular uptake of a thymidine analogue, bromodeoxyuridine (BrdU) in a primary serum-free culture system. Estradiol-17β (E2) and TGF-α treatment increased the number of BrdU-labelled mammotrophs, indicating that E2 and TGF-α treatment stimulates the DNA replication in mammotrophs. Immunoneutralization of TGF-α with anti-TGF-α-antibodies nullified the E2-induced increase in DNA replication. RT-PCR analysis of TGF-α mRNA expression in ovariectomized female mice revealed that E2 increases TGF-α mRNA levels. These results indicate that the TGF-α produced primarily in the somatotrophs mediates the stimulatory effects of estrogen on the DNA replication of pituitary cells in a paracrine or autocrine manner.",
keywords = "Estrogen, Mammotroph, Pituitary, Somatotroph, Transforming growth factor-α (TGF-α)",
author = "Seema Sharma and Souichi Oomizu and Tomoshi Kakeya and Toru Masui and Sakae Takeuchi and Sumio Takahashi",
year = "2003",
month = "1",
day = "1",
doi = "10.2108/zsj.20.83",
language = "English",
volume = "20",
pages = "83--89",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "1",

}

TY - JOUR

T1 - Gene expression and the physiological role of transforming growth factor-α in the mouse pituitary

AU - Sharma, Seema

AU - Oomizu, Souichi

AU - Kakeya, Tomoshi

AU - Masui, Toru

AU - Takeuchi, Sakae

AU - Takahashi, Sumio

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Transforming growth factor-α (TGF-α, a member of the epidermal growth factor (EGF) family, is produced within the mouse anterior pituitaries. However, the cell types of TGF-α-expressing cells and the physiological roles of TGF-α within mouse pituitary glands remain unclear. The aim of the present study was to localize TGF-α mRNA-expressing cells, and to clarify the involvement of TGF-α in estrogen-induced DNA replication in mouse anterior pituitary cells. Northern blot analysis demonstrated TGF-α mRNA expression in adult male and female mouse anterior pituitaries. In situ hybridization analysis of the pituitaries in these mice showed that TGF-α mRNA-expressing cells in the anterior pituitary are round, oval, and medium-sized. TGF-α mRNA was colocalized in most of the growth hormone (GH) mRNA-expressing cells, while only some of the prolactin (PRL) mRNA-expressing cells. DNA replication in the anterior pituitary cells was detected by monitoring the cellular uptake of a thymidine analogue, bromodeoxyuridine (BrdU) in a primary serum-free culture system. Estradiol-17β (E2) and TGF-α treatment increased the number of BrdU-labelled mammotrophs, indicating that E2 and TGF-α treatment stimulates the DNA replication in mammotrophs. Immunoneutralization of TGF-α with anti-TGF-α-antibodies nullified the E2-induced increase in DNA replication. RT-PCR analysis of TGF-α mRNA expression in ovariectomized female mice revealed that E2 increases TGF-α mRNA levels. These results indicate that the TGF-α produced primarily in the somatotrophs mediates the stimulatory effects of estrogen on the DNA replication of pituitary cells in a paracrine or autocrine manner.

AB - Transforming growth factor-α (TGF-α, a member of the epidermal growth factor (EGF) family, is produced within the mouse anterior pituitaries. However, the cell types of TGF-α-expressing cells and the physiological roles of TGF-α within mouse pituitary glands remain unclear. The aim of the present study was to localize TGF-α mRNA-expressing cells, and to clarify the involvement of TGF-α in estrogen-induced DNA replication in mouse anterior pituitary cells. Northern blot analysis demonstrated TGF-α mRNA expression in adult male and female mouse anterior pituitaries. In situ hybridization analysis of the pituitaries in these mice showed that TGF-α mRNA-expressing cells in the anterior pituitary are round, oval, and medium-sized. TGF-α mRNA was colocalized in most of the growth hormone (GH) mRNA-expressing cells, while only some of the prolactin (PRL) mRNA-expressing cells. DNA replication in the anterior pituitary cells was detected by monitoring the cellular uptake of a thymidine analogue, bromodeoxyuridine (BrdU) in a primary serum-free culture system. Estradiol-17β (E2) and TGF-α treatment increased the number of BrdU-labelled mammotrophs, indicating that E2 and TGF-α treatment stimulates the DNA replication in mammotrophs. Immunoneutralization of TGF-α with anti-TGF-α-antibodies nullified the E2-induced increase in DNA replication. RT-PCR analysis of TGF-α mRNA expression in ovariectomized female mice revealed that E2 increases TGF-α mRNA levels. These results indicate that the TGF-α produced primarily in the somatotrophs mediates the stimulatory effects of estrogen on the DNA replication of pituitary cells in a paracrine or autocrine manner.

KW - Estrogen

KW - Mammotroph

KW - Pituitary

KW - Somatotroph

KW - Transforming growth factor-α (TGF-α)

UR - http://www.scopus.com/inward/record.url?scp=0038187636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038187636&partnerID=8YFLogxK

U2 - 10.2108/zsj.20.83

DO - 10.2108/zsj.20.83

M3 - Article

C2 - 12560605

AN - SCOPUS:0038187636

VL - 20

SP - 83

EP - 89

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 1

ER -