Gene expression of interstitial collagenase (matrix metalloproteinase 1) in gastrointestinal tract cancers

Yoshihide Otani, Isao Okazaki, Masao Arai, Kaori Kameyama, Norihito Wada, Katsuya Maruyama, Keiichi Yoshino, Masaki Kitajima, Yasuhiro Hosoda, Masaharu Tsuchiya

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Abstract

Collagenolytic proteinases play an important pathological role in the invasion and metastasis of cancer cells. However, little is known about the role of interstitial collagenase (matrix metalloproteinase-1; MMP-1) in this process. To investigate the expression of the MMP-1 gene in cancer tissues, an in situ hybridization study was carried out in gastrointestinal tract cancers (one esophageal cancer, five gastric cancers, and four colorectal cancers), using a35S-labeled MMP-1 cDNA probe. The MMP-1 gene was expressed in the stromal cells of fibrous tissue around cancer nests, especially at the margin of invasion and/or within the cancer nest; however, no definite expression within cancer cells was observed. Expression of the MMP-1 gene in the stromal cells was more common in well differentiated gastric adenocarcinoma than in poorly differentiated adenocarcinomas. These findings indicate that expression of the MMP-1 gene is greater in stromal cells that are closely associated with cancer cells, suggesting a pathophysiological role of MMP-1 in the invasion and metastasis of cancer cells.

Original languageEnglish
Pages (from-to)391-397
Number of pages7
JournalJournal of Gastroenterology
Volume29
Issue number4
DOIs
Publication statusPublished - 1994 Aug

Fingerprint

Matrix Metalloproteinase 1
Gastrointestinal Neoplasms
Matrix Metalloproteinases
Gene Expression
Stromal Cells
Neoplasms
Stomach Neoplasms
Adenocarcinoma
Neoplasm Metastasis
Genes
Neoplasm Genes
Esophageal Neoplasms
In Situ Hybridization
Colorectal Neoplasms
Stomach
Peptide Hydrolases
Complementary DNA

Keywords

  • collagenase
  • gastrointestinal tract
  • in situ hybridization
  • matrix metalloproteinases
  • metastasis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Gene expression of interstitial collagenase (matrix metalloproteinase 1) in gastrointestinal tract cancers. / Otani, Yoshihide; Okazaki, Isao; Arai, Masao; Kameyama, Kaori; Wada, Norihito; Maruyama, Katsuya; Yoshino, Keiichi; Kitajima, Masaki; Hosoda, Yasuhiro; Tsuchiya, Masaharu.

In: Journal of Gastroenterology, Vol. 29, No. 4, 08.1994, p. 391-397.

Research output: Contribution to journalArticle

Otani, Y, Okazaki, I, Arai, M, Kameyama, K, Wada, N, Maruyama, K, Yoshino, K, Kitajima, M, Hosoda, Y & Tsuchiya, M 1994, 'Gene expression of interstitial collagenase (matrix metalloproteinase 1) in gastrointestinal tract cancers', Journal of Gastroenterology, vol. 29, no. 4, pp. 391-397. https://doi.org/10.1007/BF02361233
Otani, Yoshihide ; Okazaki, Isao ; Arai, Masao ; Kameyama, Kaori ; Wada, Norihito ; Maruyama, Katsuya ; Yoshino, Keiichi ; Kitajima, Masaki ; Hosoda, Yasuhiro ; Tsuchiya, Masaharu. / Gene expression of interstitial collagenase (matrix metalloproteinase 1) in gastrointestinal tract cancers. In: Journal of Gastroenterology. 1994 ; Vol. 29, No. 4. pp. 391-397.
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