Gene expression of lymphocyte prolactin receptor was suppressed in lactating mothers

Hironobu Maeda, Shun Ichiro Izumi, Yukio Kato, Li Yi Cai, Takako Kato, Takahiro Suzuki, Eri Nakamura, Taro Sugiyama, Takayo Fuda, Kazumi Takahashi, Akane Kondo, Tadashi Matsumoto, Hitoshi Ishimoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Prolactin (PRL) receptor (PRL-R) was proven to be ubiquitously expressed by cells in the immune system, while the physiological role of PRL was established in milk production in mammary glands. We analyzed the mRNA content of PRL-R in human lymphocytes in normo- and hyperprolactinemic conditions to document the presence of functioning PRL-R of human lymphocytes. Blood samples were obtained prior to treatment, and with written informed consent, from outpatients with ovarian dysfunction and hyperprolactinemia (n = 8; 19 ~ 41 y/o), from breast-feeding mothers after normal delivery (n = 12; 27 ~ 36 y/o), and from healthy volunteers: men (n = 9; 33 ~ 40 y/o) and women (n = 9; 26 ~ 36 y/o). Subsequently, total RNA was prepared from the lymphocytes separated. The quantity of PRL-R mRNA was examined by reverse transcription and polymerase chain reaction and normalized with a simultaneously measured amount of b actin. The resultant mRNA level of PRL-R was analyzed for its correlation with serum concentration of PRL measured by immunoassay. PRL-R mRNA levels of lymphocytes were significantly suppressed in lactating mothers, while there was a statistically significant negative correlation between PRL-R mRNA and serum PRL levels. However, there was no significant difference of PRL-R mRNA in the pathological condition of outpatients with ovarian dysfunction and/or hyperprolactinemia. While a few investigators reported the extra-mammary regulation on PRL-R by PRL, our data suggest that the PRL-R levels of circulating lymphocytes could be down-regulated by the elevated serum levels of PRL and that pituitary PRL may participate in regulating the expression of PRL-R genes on cells of the human immune system, especially in physiological circumstances such as in the postpartum period.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalTokai Journal of Experimental and Clinical Medicine
Volume35
Issue number2
Publication statusPublished - 2010 Jul
Externally publishedYes

Fingerprint

Prolactin Receptors
Prolactin
Mothers
Lymphocytes
Gene Expression
Messenger RNA
Hyperprolactinemia
Immune System
Outpatients
Serum
vpr Genes

Keywords

  • Human lymphocyte
  • Hyperprolactinemia
  • Lactation
  • Prolactin
  • Prolactin receptor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Maeda, H., Izumi, S. I., Kato, Y., Cai, L. Y., Kato, T., Suzuki, T., ... Ishimoto, H. (2010). Gene expression of lymphocyte prolactin receptor was suppressed in lactating mothers. Tokai Journal of Experimental and Clinical Medicine, 35(2), 62-65.

Gene expression of lymphocyte prolactin receptor was suppressed in lactating mothers. / Maeda, Hironobu; Izumi, Shun Ichiro; Kato, Yukio; Cai, Li Yi; Kato, Takako; Suzuki, Takahiro; Nakamura, Eri; Sugiyama, Taro; Fuda, Takayo; Takahashi, Kazumi; Kondo, Akane; Matsumoto, Tadashi; Ishimoto, Hitoshi.

In: Tokai Journal of Experimental and Clinical Medicine, Vol. 35, No. 2, 07.2010, p. 62-65.

Research output: Contribution to journalArticle

Maeda, H, Izumi, SI, Kato, Y, Cai, LY, Kato, T, Suzuki, T, Nakamura, E, Sugiyama, T, Fuda, T, Takahashi, K, Kondo, A, Matsumoto, T & Ishimoto, H 2010, 'Gene expression of lymphocyte prolactin receptor was suppressed in lactating mothers', Tokai Journal of Experimental and Clinical Medicine, vol. 35, no. 2, pp. 62-65.
Maeda, Hironobu ; Izumi, Shun Ichiro ; Kato, Yukio ; Cai, Li Yi ; Kato, Takako ; Suzuki, Takahiro ; Nakamura, Eri ; Sugiyama, Taro ; Fuda, Takayo ; Takahashi, Kazumi ; Kondo, Akane ; Matsumoto, Tadashi ; Ishimoto, Hitoshi. / Gene expression of lymphocyte prolactin receptor was suppressed in lactating mothers. In: Tokai Journal of Experimental and Clinical Medicine. 2010 ; Vol. 35, No. 2. pp. 62-65.
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abstract = "Prolactin (PRL) receptor (PRL-R) was proven to be ubiquitously expressed by cells in the immune system, while the physiological role of PRL was established in milk production in mammary glands. We analyzed the mRNA content of PRL-R in human lymphocytes in normo- and hyperprolactinemic conditions to document the presence of functioning PRL-R of human lymphocytes. Blood samples were obtained prior to treatment, and with written informed consent, from outpatients with ovarian dysfunction and hyperprolactinemia (n = 8; 19 ~ 41 y/o), from breast-feeding mothers after normal delivery (n = 12; 27 ~ 36 y/o), and from healthy volunteers: men (n = 9; 33 ~ 40 y/o) and women (n = 9; 26 ~ 36 y/o). Subsequently, total RNA was prepared from the lymphocytes separated. The quantity of PRL-R mRNA was examined by reverse transcription and polymerase chain reaction and normalized with a simultaneously measured amount of b actin. The resultant mRNA level of PRL-R was analyzed for its correlation with serum concentration of PRL measured by immunoassay. PRL-R mRNA levels of lymphocytes were significantly suppressed in lactating mothers, while there was a statistically significant negative correlation between PRL-R mRNA and serum PRL levels. However, there was no significant difference of PRL-R mRNA in the pathological condition of outpatients with ovarian dysfunction and/or hyperprolactinemia. While a few investigators reported the extra-mammary regulation on PRL-R by PRL, our data suggest that the PRL-R levels of circulating lymphocytes could be down-regulated by the elevated serum levels of PRL and that pituitary PRL may participate in regulating the expression of PRL-R genes on cells of the human immune system, especially in physiological circumstances such as in the postpartum period.",
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AU - Suzuki, Takahiro

AU - Nakamura, Eri

AU - Sugiyama, Taro

AU - Fuda, Takayo

AU - Takahashi, Kazumi

AU - Kondo, Akane

AU - Matsumoto, Tadashi

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