Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity

Tetsuya S. Tanaka, Tilo Kunath, Wendy L. Kimber, Saied A. Jaradat, Carole A. Stagg, Masayuki Usuda, Takashi Yokota, Hitoshi Niwa, Janet Rossant, Minoru Ko

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Large-scale gene expression profiling was performed on embryo-derived stem cell lines to identify molecular signatures of pluripotency and lineage specificity. Analysis of pluripotent embryonic stem (ES) cells, extraembryonic-restricted trophoblast stem (TS) cells, and terminally-differentiated mouse embryo fibroblast (MEF) cells identified expression profiles unique to each cell type, as well as genes common only to ES and TS cells. Whereas most of the MEF-specific genes had been characterized previously, the majority (67%) of the ES-specific genes were novel and did not include known differentiated cell markers. Comparison with microarray data from embryonic material demonstrated that ES-specific genes were underrepresented in all stages sampled, whereas TS-specific genes included known placental markers. Investigation of four novel TS-specific genes showed trophoblast-restricted expression in cell lines and in vivo, whereas one uncharacterized ES-specific gene, Esg-1, was found to be exclusively associated with pluripotency. We suggest that pluripotency requires a set of genes not expressed in other cell types, whereas lineage-restricted stem cells, like TS cells, express genes predictive of their differentiated lineage.

Original languageEnglish
Pages (from-to)1921-1928
Number of pages8
JournalGenome Research
Volume12
Issue number12
DOIs
Publication statusPublished - 2002 Dec 1
Externally publishedYes

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Gene Expression Profiling
Stem Cells
Embryonic Structures
Trophoblasts
Genes
Fibroblasts
Cell Line
Pluripotent Stem Cells
Cell Lineage
Embryonic Stem Cells

ASJC Scopus subject areas

  • Genetics

Cite this

Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity. / Tanaka, Tetsuya S.; Kunath, Tilo; Kimber, Wendy L.; Jaradat, Saied A.; Stagg, Carole A.; Usuda, Masayuki; Yokota, Takashi; Niwa, Hitoshi; Rossant, Janet; Ko, Minoru.

In: Genome Research, Vol. 12, No. 12, 01.12.2002, p. 1921-1928.

Research output: Contribution to journalArticle

Tanaka, TS, Kunath, T, Kimber, WL, Jaradat, SA, Stagg, CA, Usuda, M, Yokota, T, Niwa, H, Rossant, J & Ko, M 2002, 'Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity', Genome Research, vol. 12, no. 12, pp. 1921-1928. https://doi.org/10.1101/gr.670002
Tanaka, Tetsuya S. ; Kunath, Tilo ; Kimber, Wendy L. ; Jaradat, Saied A. ; Stagg, Carole A. ; Usuda, Masayuki ; Yokota, Takashi ; Niwa, Hitoshi ; Rossant, Janet ; Ko, Minoru. / Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity. In: Genome Research. 2002 ; Vol. 12, No. 12. pp. 1921-1928.
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