Gene expression profiling of mouse embryos with microarrays

Alexei A. Sharov, Yulan Piao, Minoru Ko

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Global expression profiling by DNA microarrays provides a snapshot of cell and tissue status and becomes an essential tool in biological and medical sciences. Typical questions that can be addressed by microarray analysis in developmental biology include: (1) to find a set of genes expressed in a specific cell type; (2) to identify genes expressed commonly in multiple cell types; (3) to follow the time-course changes of gene expression patterns; (4) to demonstrate cell's identity by showing similarities or differences among two or multiple cell types; (5) to find regulatory pathways and/or networks affected by gene manipulations, such as overexpression or repression of gene expression; (6) to find downstream target genes of transcription factors; (7) to find downstream target genes of cell signaling; (8) to examine the effects of environmental manipulation of cells on gene expression patterns; and (9) to find the effects of genetic manipulation in embryos and adults. Here, we describe strategies for executing these experiments and monitoring changes of cell state with gene expression microarrays in application to mouse embryology. Both statistical assessment and interpretation of data are discussed. We also present a protocol for performing microarray analysis on a small amount of embryonic materials.

Original languageEnglish
Pages (from-to)511-541
Number of pages31
JournalMethods in Enzymology
Volume477
Issue numberC
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Gene Expression Profiling
Microarrays
Gene expression
Embryonic Structures
Genes
Gene Expression
Cell signaling
Microarray Analysis
Developmental Biology
Transcription Factors
Statistical Data Interpretation
Embryology
Biological Science Disciplines
Gene Regulatory Networks
Tissue
Oligonucleotide Array Sequence Analysis
Monitoring
DNA
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Gene expression profiling of mouse embryos with microarrays. / Sharov, Alexei A.; Piao, Yulan; Ko, Minoru.

In: Methods in Enzymology, Vol. 477, No. C, 2010, p. 511-541.

Research output: Contribution to journalArticle

Sharov, Alexei A. ; Piao, Yulan ; Ko, Minoru. / Gene expression profiling of mouse embryos with microarrays. In: Methods in Enzymology. 2010 ; Vol. 477, No. C. pp. 511-541.
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