Gene expression profiling of synovial sarcoma: Distinct signature of poorly differentiated type

Turrent Robert Nakayama, Sachiyo Mitani, Takeshi Nakagawa, Tadashi Hasegawa, Akira Kawai, Hideo Morioka, Hiroo Yabe, Yoshiaki Toyama, Akira Ogose, Junya Toguchida, Tomitaka Nakayama, Teruhiko Yoshida, Hitoshi Ichikawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Poorly differentiated type synovial sarcoma (PDSS) is a variant of synovial sarcoma characterized by predominantly round or short-spindled cell morphology. Although accumulating evidence from clinicopathologic studies suggests a strong association between this variant of synovial sarcoma and poor prognosis, little has been reported on the molecular basis of PDSS. To gain insights into the mechanism(s) that underlie the emergence of PDSS, we analyzed the gene expression profiles of 34 synovial sarcoma clinical samples, including 5 cases of PDSS, using an oligonucleotide microarray. In an unsupervised analysis, the 34 samples fell into 3 groups that correlate closely with histologic subtypes: monophasic, biphasic, and poorly differentiated types. PDSS was characterized by down-regulation of genes associated with neuronal and skeletal development and cell adhesion. Moreover, upregulation of genes on a specific chromosomal locus, 8q21.11, was identified. This locus-specific transcriptional activation in PDSS was confirmed by reverse transcriptase-PCR analysis of 9 additional synovial sarcoma samples. Our results indicate that PDSS tumors constitute a distinct group based on expression profiles.

Original languageEnglish
Pages (from-to)1599-1607
Number of pages9
JournalAmerican Journal of Surgical Pathology
Volume34
Issue number11
DOIs
Publication statusPublished - 2010 Nov

Fingerprint

Synovial Sarcoma
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Transcriptome
Cell Adhesion
Transcriptional Activation
Genes
Up-Regulation
Down-Regulation

Keywords

  • gene expression profiling
  • poorly differentiated type
  • synovial sarcoma

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Surgery

Cite this

Gene expression profiling of synovial sarcoma : Distinct signature of poorly differentiated type. / Nakayama, Turrent Robert; Mitani, Sachiyo; Nakagawa, Takeshi; Hasegawa, Tadashi; Kawai, Akira; Morioka, Hideo; Yabe, Hiroo; Toyama, Yoshiaki; Ogose, Akira; Toguchida, Junya; Nakayama, Tomitaka; Yoshida, Teruhiko; Ichikawa, Hitoshi.

In: American Journal of Surgical Pathology, Vol. 34, No. 11, 11.2010, p. 1599-1607.

Research output: Contribution to journalArticle

Nakayama, TR, Mitani, S, Nakagawa, T, Hasegawa, T, Kawai, A, Morioka, H, Yabe, H, Toyama, Y, Ogose, A, Toguchida, J, Nakayama, T, Yoshida, T & Ichikawa, H 2010, 'Gene expression profiling of synovial sarcoma: Distinct signature of poorly differentiated type', American Journal of Surgical Pathology, vol. 34, no. 11, pp. 1599-1607. https://doi.org/10.1097/PAS.0b013e3181f7ce2c
Nakayama, Turrent Robert ; Mitani, Sachiyo ; Nakagawa, Takeshi ; Hasegawa, Tadashi ; Kawai, Akira ; Morioka, Hideo ; Yabe, Hiroo ; Toyama, Yoshiaki ; Ogose, Akira ; Toguchida, Junya ; Nakayama, Tomitaka ; Yoshida, Teruhiko ; Ichikawa, Hitoshi. / Gene expression profiling of synovial sarcoma : Distinct signature of poorly differentiated type. In: American Journal of Surgical Pathology. 2010 ; Vol. 34, No. 11. pp. 1599-1607.
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