Gene expression waves

Cell cycle independent collective dynamics in cultured cells

Masa Tsuchyia, Sum T. Wong, Zhen X. Yeo, Alfredo Colosimo, Maria C. Palumbo, Lorenzo Farina, Marco Crescenzi, Alessia Mazzola, Rodolfo Negri, Michele M. Bianchi, Kumar Selvarajoo, Masaru Tomita, Alessandro Giuliani

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The ergodic hypothesis, which assumes the independence of each cell of the ensemble from all the others, is a necessary prerequisite to attach single cell based explanations to the grand averages taken from population data. This was the prevailing view about the interpretation of cellular biology experiments that typically are performed on colonies of billions of cells. By analysing gene expression data of different cells going from yeast to mammalian cell cultures, we demonstrate that cell cultures display a sort of 'ecology-in-a-plate' giving rise to a rich dynamics of gene expression that are independent from reproductive cycles, hence contradicting simple ergodic assumptions The aspecific character of the observed coordinated gene expression activity inhibits any simple mechanistic hypothesis and highlights the need to consider population effects in the interpretation of data coming from cell cultures.

Original languageEnglish
Pages (from-to)2878-2886
Number of pages9
JournalFEBS Journal
Volume274
Issue number11
DOIs
Publication statusPublished - 2007 Jun

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Cell culture
Gene expression
Cultured Cells
Cell Cycle
Cells
Gene Expression
Cell Culture Techniques
Cytology
Ecology
Yeast
Population
Cell Biology
Yeasts
Experiments

Keywords

  • Cell-cell communication
  • Metabolic cycle
  • Microarray
  • Ribogenesis cycle
  • Systems biology

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tsuchyia, M., Wong, S. T., Yeo, Z. X., Colosimo, A., Palumbo, M. C., Farina, L., ... Giuliani, A. (2007). Gene expression waves: Cell cycle independent collective dynamics in cultured cells. FEBS Journal, 274(11), 2878-2886. https://doi.org/10.1111/j.1742-4658.2007.05822.x

Gene expression waves : Cell cycle independent collective dynamics in cultured cells. / Tsuchyia, Masa; Wong, Sum T.; Yeo, Zhen X.; Colosimo, Alfredo; Palumbo, Maria C.; Farina, Lorenzo; Crescenzi, Marco; Mazzola, Alessia; Negri, Rodolfo; Bianchi, Michele M.; Selvarajoo, Kumar; Tomita, Masaru; Giuliani, Alessandro.

In: FEBS Journal, Vol. 274, No. 11, 06.2007, p. 2878-2886.

Research output: Contribution to journalArticle

Tsuchyia, M, Wong, ST, Yeo, ZX, Colosimo, A, Palumbo, MC, Farina, L, Crescenzi, M, Mazzola, A, Negri, R, Bianchi, MM, Selvarajoo, K, Tomita, M & Giuliani, A 2007, 'Gene expression waves: Cell cycle independent collective dynamics in cultured cells', FEBS Journal, vol. 274, no. 11, pp. 2878-2886. https://doi.org/10.1111/j.1742-4658.2007.05822.x
Tsuchyia M, Wong ST, Yeo ZX, Colosimo A, Palumbo MC, Farina L et al. Gene expression waves: Cell cycle independent collective dynamics in cultured cells. FEBS Journal. 2007 Jun;274(11):2878-2886. https://doi.org/10.1111/j.1742-4658.2007.05822.x
Tsuchyia, Masa ; Wong, Sum T. ; Yeo, Zhen X. ; Colosimo, Alfredo ; Palumbo, Maria C. ; Farina, Lorenzo ; Crescenzi, Marco ; Mazzola, Alessia ; Negri, Rodolfo ; Bianchi, Michele M. ; Selvarajoo, Kumar ; Tomita, Masaru ; Giuliani, Alessandro. / Gene expression waves : Cell cycle independent collective dynamics in cultured cells. In: FEBS Journal. 2007 ; Vol. 274, No. 11. pp. 2878-2886.
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