Gene induction in mature oligodendrocytes with a PLP-tTA mouse line

Naoko Inamura, Shouta Sugio, Wendy B. Macklin, Koichi Tomita, Kenji F. Tanaka, Kazuhiro Ikenaka

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Mature oligodendrocytes are critical for myelin maintenance. To understand the molecular basis for this, genetic manipulation of mature oligodendrocytes is needed. Here we generated a mature oligodendrocyte tTA (tetracycline-controlled transcriptional activator) mouse line which, in combination with a tTA-dependent promoter line driving the expression of the desired transgene, can be used for gain-of-function studies. We used an oligodendrocyte promoter, the mouse proteolipid protein (PLP) promoter, to express mammalianized tTA, and generated a PLP-mtTA mouse line. In adults, mtTA mRNA was predominantly detected in brain white matter where it co-localized with PLP mRNA. mtTA-mediated gene induction was confirmed by crossing to mice with a tTA-dependent promoter driving expression of yellow fluorescent protein (tetO-YFP mice). YFP induction in PLP-mtTA::tetO-YFP mice was consistent with PLP expression in adult mature oligodendrocytes and premyelinating-stage myelinating oligodendrocytes. This PLP-mtTA mouse line is the first to enable gain-of-function studies in mature oligodendrocytes with the tet system.

Original languageEnglish
Pages (from-to)424-428
Number of pages5
JournalGenesis
Volume50
Issue number5
DOIs
Publication statusPublished - 2012 May

Keywords

  • Myelinating
  • Proteolipid protein
  • Transactivation

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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