Gene induction in mature oligodendrocytes with a PLP-tTA mouse line

Naoko Inamura, Shouta Sugio, Wendy B. Macklin, Koichi Tomita, Kenji Tanaka, Kazuhiro Ikenaka

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Mature oligodendrocytes are critical for myelin maintenance. To understand the molecular basis for this, genetic manipulation of mature oligodendrocytes is needed. Here we generated a mature oligodendrocyte tTA (tetracycline-controlled transcriptional activator) mouse line which, in combination with a tTA-dependent promoter line driving the expression of the desired transgene, can be used for gain-of-function studies. We used an oligodendrocyte promoter, the mouse proteolipid protein (PLP) promoter, to express mammalianized tTA, and generated a PLP-mtTA mouse line. In adults, mtTA mRNA was predominantly detected in brain white matter where it co-localized with PLP mRNA. mtTA-mediated gene induction was confirmed by crossing to mice with a tTA-dependent promoter driving expression of yellow fluorescent protein (tetO-YFP mice). YFP induction in PLP-mtTA::tetO-YFP mice was consistent with PLP expression in adult mature oligodendrocytes and premyelinating-stage myelinating oligodendrocytes. This PLP-mtTA mouse line is the first to enable gain-of-function studies in mature oligodendrocytes with the tet system.

Original languageEnglish
Pages (from-to)424-428
Number of pages5
JournalGenesis
Volume50
Issue number5
DOIs
Publication statusPublished - 2012 May
Externally publishedYes

Fingerprint

Proteolipids
Oligodendroglia
Tetracycline
Genes
Proteins
Messenger RNA
Myelin Sheath
Transgenes
Molecular Biology
Maintenance
Brain

Keywords

  • Myelinating
  • Proteolipid protein
  • Transactivation

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics

Cite this

Inamura, N., Sugio, S., Macklin, W. B., Tomita, K., Tanaka, K., & Ikenaka, K. (2012). Gene induction in mature oligodendrocytes with a PLP-tTA mouse line. Genesis, 50(5), 424-428. https://doi.org/10.1002/dvg.20808

Gene induction in mature oligodendrocytes with a PLP-tTA mouse line. / Inamura, Naoko; Sugio, Shouta; Macklin, Wendy B.; Tomita, Koichi; Tanaka, Kenji; Ikenaka, Kazuhiro.

In: Genesis, Vol. 50, No. 5, 05.2012, p. 424-428.

Research output: Contribution to journalArticle

Inamura, N, Sugio, S, Macklin, WB, Tomita, K, Tanaka, K & Ikenaka, K 2012, 'Gene induction in mature oligodendrocytes with a PLP-tTA mouse line', Genesis, vol. 50, no. 5, pp. 424-428. https://doi.org/10.1002/dvg.20808
Inamura N, Sugio S, Macklin WB, Tomita K, Tanaka K, Ikenaka K. Gene induction in mature oligodendrocytes with a PLP-tTA mouse line. Genesis. 2012 May;50(5):424-428. https://doi.org/10.1002/dvg.20808
Inamura, Naoko ; Sugio, Shouta ; Macklin, Wendy B. ; Tomita, Koichi ; Tanaka, Kenji ; Ikenaka, Kazuhiro. / Gene induction in mature oligodendrocytes with a PLP-tTA mouse line. In: Genesis. 2012 ; Vol. 50, No. 5. pp. 424-428.
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