Gene induction in mature oligodendrocytes with a PLP-tTA mouse line

Naoko Inamura, Shouta Sugio, Wendy B. Macklin, Koichi Tomita, Kenji F. Tanaka, Kazuhiro Ikenaka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mature oligodendrocytes are critical for myelin maintenance. To understand the molecular basis for this, genetic manipulation of mature oligodendrocytes is needed. Here we generated a mature oligodendrocyte tTA (tetracycline-controlled transcriptional activator) mouse line which, in combination with a tTA-dependent promoter line driving the expression of the desired transgene, can be used for gain-of-function studies. We used an oligodendrocyte promoter, the mouse proteolipid protein (PLP) promoter, to express mammalianized tTA, and generated a PLP-mtTA mouse line. In adults, mtTA mRNA was predominantly detected in brain white matter where it co-localized with PLP mRNA. mtTA-mediated gene induction was confirmed by crossing to mice with a tTA-dependent promoter driving expression of yellow fluorescent protein (tetO-YFP mice). YFP induction in PLP-mtTA::tetO-YFP mice was consistent with PLP expression in adult mature oligodendrocytes and premyelinating-stage myelinating oligodendrocytes. This PLP-mtTA mouse line is the first to enable gain-of-function studies in mature oligodendrocytes with the tet system.

Original languageEnglish
Pages (from-to)424-428
Number of pages5
JournalGenesis
Volume50
Issue number5
DOIs
Publication statusPublished - 2012 May 1
Externally publishedYes

Keywords

  • Myelinating
  • Proteolipid protein
  • Transactivation

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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    Inamura, N., Sugio, S., Macklin, W. B., Tomita, K., Tanaka, K. F., & Ikenaka, K. (2012). Gene induction in mature oligodendrocytes with a PLP-tTA mouse line. Genesis, 50(5), 424-428. https://doi.org/10.1002/dvg.20808