Gene knockout analysis of two γ-tubulin isoforms in mice

Akiko Kubo, Akiharu Kubo, Masaki Hata, Shoichiro Tsukita

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Abstract

γ-Tubulin regulates the nucleation of microtubules, but knowledge of its functions in vivo is still fragmentary. Here, we report the identification of two closely related γ-tubulin isoforms, TUBG1 and TUBG2, in mice, and the generation of TUBG1- and TUBG2-deficient mice. TUBG1 was expressed ubiquitously, whereas TUBG2 was primarily detected in the brain. The development of TUBG1-deficient (Tubg1-/-) embryos stopped at the morula/blastocyst stages due to a characteristic mitotic arrest: the mitotic spindle was highly disorganized, and disorganized spindles showed one or two pole-like foci of bundled MTs that were surrounded by condensed chromosomes. TUBG2 was expressed in blastocysts, but could not rescue the TUBG1 deficiency. By contrast, TUBG2-deficient (Tubg2-/-) mice were born, grew, and intercrossed normally. In the brain of wild-type mice, TUBG2 was expressed in approximately the same amount as TUBG1, but no histological abnormalities were found in the Tubg2-/- brain. These findings indicated that TUBG1 and TUBG2 are not functionally equivalent in vivo, that TUBG1 corresponds to conventional γ-tubulin, and that TUBG2 may have some unidentified function in the brain.

Original languageEnglish
Pages (from-to)361-373
Number of pages13
JournalDevelopmental Biology
Volume282
Issue number2
DOIs
Publication statusPublished - 2005 Jun 15
Externally publishedYes

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Keywords

  • γ-Tubulin
  • Gene knockout
  • Isoform
  • Microtubule
  • Mitotic spindle

ASJC Scopus subject areas

  • Developmental Biology

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