Gene networks involved in apoptosis induced by hyperthermia in human lymphoma U937 cells

Yukihiro Furusawa, Yoshiaki Tabuchi, Ichiro Takasaki, Shigehito Wada, Kenzo Ohtsuka, Takashi Kondo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To define the molecular mechanisms that mediate hyperthermia-induced apoptosis, we performed microarray and computational gene expression analyses. U937 cells, a human myelomonocytic lymphoma cell line, were treated with hyperthermia at 42 °C for 90 min and cultured at 37 °C. Apoptotic cells (∼15%) were seen 6 h after hyperthermic treatment, and elevated expression of heat shock proteins (HSPs) including Hsp27, Hsp40, and Hsp70 was detected, following the activation of heat shock factor-1. Of the 54,675 probe sets analyzed, 1334 were upregulated and 4214 were downregulated by >2.0-fold in the cells treated with hyperthermia. A non-hierarchical gene clustering algorithm, K-means clustering, demonstrated 10 gene clusters. The gene network U1 or U2 that was obtained from up-regulated genes in cluster I or IX contained HSPA1B, DNAJB1, HSPH1, and TXN or PML, LYN, and DUSP1, and were mainly associated with cellular compromise, and cellular function and maintenance or death, and cancer, respectively. In the decreased gene cluster II, the gene network D1 including CCNE1 and CEBPE was associated with the cell cycle and cellular growth and proliferation. These findings will provide a basis for understanding the detailed molecular mechanisms of apoptosis induced by hyperthermia at 42 °C in cells.

Original languageEnglish
Pages (from-to)1253-1262
Number of pages10
JournalCell Biology International
Volume33
Issue number12
DOIs
Publication statusPublished - 2009 Dec

Fingerprint

U937 Cells
Induced Hyperthermia
Gene Regulatory Networks
Multigene Family
Lymphoma
Apoptosis
Cluster Analysis
Fever
Heat-Shock Proteins
Shock
Cell Cycle
Down-Regulation
Hot Temperature
Maintenance
Cell Proliferation
Gene Expression
Cell Line
Growth
Genes
Neoplasms

Keywords

  • Apoptosis
  • Gene expression
  • Gene network
  • Heat shock protein
  • Hyperthermia

ASJC Scopus subject areas

  • Cell Biology

Cite this

Furusawa, Y., Tabuchi, Y., Takasaki, I., Wada, S., Ohtsuka, K., & Kondo, T. (2009). Gene networks involved in apoptosis induced by hyperthermia in human lymphoma U937 cells. Cell Biology International, 33(12), 1253-1262. https://doi.org/10.1016/j.cellbi.2009.08.009

Gene networks involved in apoptosis induced by hyperthermia in human lymphoma U937 cells. / Furusawa, Yukihiro; Tabuchi, Yoshiaki; Takasaki, Ichiro; Wada, Shigehito; Ohtsuka, Kenzo; Kondo, Takashi.

In: Cell Biology International, Vol. 33, No. 12, 12.2009, p. 1253-1262.

Research output: Contribution to journalArticle

Furusawa, Y, Tabuchi, Y, Takasaki, I, Wada, S, Ohtsuka, K & Kondo, T 2009, 'Gene networks involved in apoptosis induced by hyperthermia in human lymphoma U937 cells', Cell Biology International, vol. 33, no. 12, pp. 1253-1262. https://doi.org/10.1016/j.cellbi.2009.08.009
Furusawa, Yukihiro ; Tabuchi, Yoshiaki ; Takasaki, Ichiro ; Wada, Shigehito ; Ohtsuka, Kenzo ; Kondo, Takashi. / Gene networks involved in apoptosis induced by hyperthermia in human lymphoma U937 cells. In: Cell Biology International. 2009 ; Vol. 33, No. 12. pp. 1253-1262.
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