Gene organization and transcription of duplicated MBP genes of myelin deficient (shi(mld)) mutant mouse.

Hideyuki Okano, T. Tamura, M. Miura, A. Aoyama, K. Ikenaka, M. Oshimura, K. Mikoshiba

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A hereditary dysmyelinating mutation, named myelin deficient (shi(mld)), is characterized by reduced expression of myelin basic protein (MBP). In shi(mld), the MBP gene is duplicated and its reduced expression is mainly determined by the level of mRNA. We have characterized the structure and function of the promoter regions of the duplicated MBP genes in shi(mld). Among the lambda clones containing promoter regions of the duplicated MBP genes in shi(mld), one (gene 1) had the same restriction enzyme pattern as that in control mice, but another (gene 2) had a rearrangement on a distal part of the promoter. A 712-bp nucleotide sequence upstream of the first exons of both of the duplicated MBP genes of shi(mld) was completely consistent with that of the control. Promoter activities of 1.3-kb 5'-flanking regions from respective genes of shi(mld) measured by in vitro run-off assay using HeLa whole-cell extracts were indistinguishable from that of the control MPB gene. Chromosomal mapping by in situ hybridization suggested that the duplicated MBP genes were located closely to each other at the distal part of chromosome 18. A recombinational event including the inversion seemed to have occurred within gene 1 and its possible relationship to the reduced expression of MBP is discussed.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalEMBO Journal
Volume7
Issue number1
Publication statusPublished - 1988 Jan
Externally publishedYes

Fingerprint

Myelin Basic Protein
Transcription
Myelin Sheath
Genes
Genetic Promoter Regions
Chromosomes, Human, Pair 18
5' Flanking Region
Chromosomes
Cell Extracts
HeLa Cells
In Situ Hybridization
Exons
Assays
Nucleotides
Clone Cells
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Okano, H., Tamura, T., Miura, M., Aoyama, A., Ikenaka, K., Oshimura, M., & Mikoshiba, K. (1988). Gene organization and transcription of duplicated MBP genes of myelin deficient (shi(mld)) mutant mouse. EMBO Journal, 7(1), 77-83.

Gene organization and transcription of duplicated MBP genes of myelin deficient (shi(mld)) mutant mouse. / Okano, Hideyuki; Tamura, T.; Miura, M.; Aoyama, A.; Ikenaka, K.; Oshimura, M.; Mikoshiba, K.

In: EMBO Journal, Vol. 7, No. 1, 01.1988, p. 77-83.

Research output: Contribution to journalArticle

Okano, H, Tamura, T, Miura, M, Aoyama, A, Ikenaka, K, Oshimura, M & Mikoshiba, K 1988, 'Gene organization and transcription of duplicated MBP genes of myelin deficient (shi(mld)) mutant mouse.', EMBO Journal, vol. 7, no. 1, pp. 77-83.
Okano H, Tamura T, Miura M, Aoyama A, Ikenaka K, Oshimura M et al. Gene organization and transcription of duplicated MBP genes of myelin deficient (shi(mld)) mutant mouse. EMBO Journal. 1988 Jan;7(1):77-83.
Okano, Hideyuki ; Tamura, T. ; Miura, M. ; Aoyama, A. ; Ikenaka, K. ; Oshimura, M. ; Mikoshiba, K. / Gene organization and transcription of duplicated MBP genes of myelin deficient (shi(mld)) mutant mouse. In: EMBO Journal. 1988 ; Vol. 7, No. 1. pp. 77-83.
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