Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad

Kazumichi M. Nishida, Kuniaki Saito, Tomoko Mori, Yoshinori Kawamura, Tomoko Nagami-Okada, Sachi Inagaki, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Genetic studies have shown that Aubergine (Aub), one of the Piwi subfamily of Argonautes in Drosophila, is essential for germ cell formation and maintaining fertility. aub mutations lead to the accumulation of retrotransposons in ovaries and testes, and Stellate transcripts in testes. Aub in ovaries associates with a variety of Piwi-interacting RNAs (piRNAs) derived from repetitive intergenic elements including retrotransposons. Here we found that Aub in testes also associates with various kinds of piRNAs. Although in ovaries Aub-associated piRNA populations are quite diverse, piRNAs with Aub in testes show a strong bias. The most abundant piRNAs were those corresponding to antisense transcripts of Suppressor of Stellate [Su(Ste)] genes known to be involved in Stellate gene silencing. The second most abundant class was made up of those from chromosome X and showed strong complementarity to vasa transcripts. Immunopurified Aub-piRNA complexes from testes displayed activity in cleaving target RNA containing sequences complementary to Stellate and vasa transcripts. These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish
Pages (from-to)1911-1922
Number of pages12
JournalRNA
Volume13
Issue number11
DOIs
Publication statusPublished - 2007 Nov
Externally publishedYes

Fingerprint

Solanum melongena
Gonads
Gene Silencing
Small Interfering RNA
Drosophila
Testis
Ovary
Retroelements
Suppressor Genes
X Chromosome
Germ Cells
Diptera
Fertility
Mutation

Keywords

  • Aubergine
  • Germlines
  • piRNA
  • RNA silencing
  • Stellate

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Nishida, K. M., Saito, K., Mori, T., Kawamura, Y., Nagami-Okada, T., Inagaki, S., ... Siomi, M. C. (2007). Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad. RNA, 13(11), 1911-1922. https://doi.org/10.1261/rna.744307

Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad. / Nishida, Kazumichi M.; Saito, Kuniaki; Mori, Tomoko; Kawamura, Yoshinori; Nagami-Okada, Tomoko; Inagaki, Sachi; Siomi, Haruhiko; Siomi, Mikiko C.

In: RNA, Vol. 13, No. 11, 11.2007, p. 1911-1922.

Research output: Contribution to journalArticle

Nishida, KM, Saito, K, Mori, T, Kawamura, Y, Nagami-Okada, T, Inagaki, S, Siomi, H & Siomi, MC 2007, 'Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad', RNA, vol. 13, no. 11, pp. 1911-1922. https://doi.org/10.1261/rna.744307
Nishida KM, Saito K, Mori T, Kawamura Y, Nagami-Okada T, Inagaki S et al. Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad. RNA. 2007 Nov;13(11):1911-1922. https://doi.org/10.1261/rna.744307
Nishida, Kazumichi M. ; Saito, Kuniaki ; Mori, Tomoko ; Kawamura, Yoshinori ; Nagami-Okada, Tomoko ; Inagaki, Sachi ; Siomi, Haruhiko ; Siomi, Mikiko C. / Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad. In: RNA. 2007 ; Vol. 13, No. 11. pp. 1911-1922.
@article{f71facd343114a69a8de59d0633b5a11,
title = "Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad",
abstract = "Genetic studies have shown that Aubergine (Aub), one of the Piwi subfamily of Argonautes in Drosophila, is essential for germ cell formation and maintaining fertility. aub mutations lead to the accumulation of retrotransposons in ovaries and testes, and Stellate transcripts in testes. Aub in ovaries associates with a variety of Piwi-interacting RNAs (piRNAs) derived from repetitive intergenic elements including retrotransposons. Here we found that Aub in testes also associates with various kinds of piRNAs. Although in ovaries Aub-associated piRNA populations are quite diverse, piRNAs with Aub in testes show a strong bias. The most abundant piRNAs were those corresponding to antisense transcripts of Suppressor of Stellate [Su(Ste)] genes known to be involved in Stellate gene silencing. The second most abundant class was made up of those from chromosome X and showed strong complementarity to vasa transcripts. Immunopurified Aub-piRNA complexes from testes displayed activity in cleaving target RNA containing sequences complementary to Stellate and vasa transcripts. These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes. Published by Cold Spring Harbor Laboratory Press.",
keywords = "Aubergine, Germlines, piRNA, RNA silencing, Stellate",
author = "Nishida, {Kazumichi M.} and Kuniaki Saito and Tomoko Mori and Yoshinori Kawamura and Tomoko Nagami-Okada and Sachi Inagaki and Haruhiko Siomi and Siomi, {Mikiko C.}",
year = "2007",
month = "11",
doi = "10.1261/rna.744307",
language = "English",
volume = "13",
pages = "1911--1922",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "11",

}

TY - JOUR

T1 - Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad

AU - Nishida, Kazumichi M.

AU - Saito, Kuniaki

AU - Mori, Tomoko

AU - Kawamura, Yoshinori

AU - Nagami-Okada, Tomoko

AU - Inagaki, Sachi

AU - Siomi, Haruhiko

AU - Siomi, Mikiko C.

PY - 2007/11

Y1 - 2007/11

N2 - Genetic studies have shown that Aubergine (Aub), one of the Piwi subfamily of Argonautes in Drosophila, is essential for germ cell formation and maintaining fertility. aub mutations lead to the accumulation of retrotransposons in ovaries and testes, and Stellate transcripts in testes. Aub in ovaries associates with a variety of Piwi-interacting RNAs (piRNAs) derived from repetitive intergenic elements including retrotransposons. Here we found that Aub in testes also associates with various kinds of piRNAs. Although in ovaries Aub-associated piRNA populations are quite diverse, piRNAs with Aub in testes show a strong bias. The most abundant piRNAs were those corresponding to antisense transcripts of Suppressor of Stellate [Su(Ste)] genes known to be involved in Stellate gene silencing. The second most abundant class was made up of those from chromosome X and showed strong complementarity to vasa transcripts. Immunopurified Aub-piRNA complexes from testes displayed activity in cleaving target RNA containing sequences complementary to Stellate and vasa transcripts. These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes. Published by Cold Spring Harbor Laboratory Press.

AB - Genetic studies have shown that Aubergine (Aub), one of the Piwi subfamily of Argonautes in Drosophila, is essential for germ cell formation and maintaining fertility. aub mutations lead to the accumulation of retrotransposons in ovaries and testes, and Stellate transcripts in testes. Aub in ovaries associates with a variety of Piwi-interacting RNAs (piRNAs) derived from repetitive intergenic elements including retrotransposons. Here we found that Aub in testes also associates with various kinds of piRNAs. Although in ovaries Aub-associated piRNA populations are quite diverse, piRNAs with Aub in testes show a strong bias. The most abundant piRNAs were those corresponding to antisense transcripts of Suppressor of Stellate [Su(Ste)] genes known to be involved in Stellate gene silencing. The second most abundant class was made up of those from chromosome X and showed strong complementarity to vasa transcripts. Immunopurified Aub-piRNA complexes from testes displayed activity in cleaving target RNA containing sequences complementary to Stellate and vasa transcripts. These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes. Published by Cold Spring Harbor Laboratory Press.

KW - Aubergine

KW - Germlines

KW - piRNA

KW - RNA silencing

KW - Stellate

UR - http://www.scopus.com/inward/record.url?scp=35548991887&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35548991887&partnerID=8YFLogxK

U2 - 10.1261/rna.744307

DO - 10.1261/rna.744307

M3 - Article

VL - 13

SP - 1911

EP - 1922

JO - RNA

JF - RNA

SN - 1355-8382

IS - 11

ER -