Abstract
Chitosan is one of naturally occurring polycations and is useful as a non-viral vector for gene delivery into cells. As PEG-C is carboxylic acid-pendant poly(ethylene glycol) derivatives, the negatively charged PEG-C can coat the surface of positively charged plasmid/chitosan complexes. The pDNA/chitosan complexes coated with the lactose modified PEG-C showed the high gene expression. To analyze the cell transfection mechanism, the amount of cell uptake was determined using a FACS, and intracellular transfer of the complexes was observed using a confocal laser scanning microscope (CLSM). The amount of cell uptake did not correlate with the gene expression, but the cell uptake of lactose-bearing complexes is faster than conventional PEG-C complexes. The CSLM showed that the pDNA/chitosan/PEG-C and pDNA/chitosan/Lac-PEG-C complexes were taken up into cells without dissociation. Moreover, the pDNA/chitosan and pDNA/chitosan/Lac-PEG-C complexes showed nuclear import after eight hours.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - 2005 Dec 1 |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country/Territory | Japan |
| City | Yokohama |
| Period | 05/5/25 → 05/5/27 |
Keywords
- Carbohydrate recognition
- Chitosan
- Confocal laser scanning microscope
- FACS
- Gene transfer
- PEG
ASJC Scopus subject areas
- Engineering(all)