Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie, Masazumi Eriguchi, Toshinori Sato

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

Original languageEnglish
Pages (from-to)815-821
Number of pages7
JournalBiotechnology Letters
Volume28
Issue number11
DOIs
Publication statusPublished - 2006 Jun

Fingerprint

Gene transfer
Macrophages
Chitosan
Peritoneal Macrophages
DNA
Mannose
Genes
Cytotoxicity
Transfection
Polyetherimides
Polymers
Lasers
Microscopes
Observation

Keywords

  • Chitosan
  • Gene transfer
  • Macrophage
  • Mannose
  • Plasmid DNA
  • Receptor-mediated endocytosis

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Hashimoto, M., Morimoto, M., Saimoto, H., Shigemasa, Y., Yanagie, H., Eriguchi, M., & Sato, T. (2006). Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages. Biotechnology Letters, 28(11), 815-821. https://doi.org/10.1007/s10529-006-9006-x

Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages. / Hashimoto, Mayu; Morimoto, Minoru; Saimoto, Hiroyuki; Shigemasa, Yoshihiro; Yanagie, Hironobu; Eriguchi, Masazumi; Sato, Toshinori.

In: Biotechnology Letters, Vol. 28, No. 11, 06.2006, p. 815-821.

Research output: Contribution to journalArticle

Hashimoto, M, Morimoto, M, Saimoto, H, Shigemasa, Y, Yanagie, H, Eriguchi, M & Sato, T 2006, 'Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages', Biotechnology Letters, vol. 28, no. 11, pp. 815-821. https://doi.org/10.1007/s10529-006-9006-x
Hashimoto M, Morimoto M, Saimoto H, Shigemasa Y, Yanagie H, Eriguchi M et al. Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages. Biotechnology Letters. 2006 Jun;28(11):815-821. https://doi.org/10.1007/s10529-006-9006-x
Hashimoto, Mayu ; Morimoto, Minoru ; Saimoto, Hiroyuki ; Shigemasa, Yoshihiro ; Yanagie, Hironobu ; Eriguchi, Masazumi ; Sato, Toshinori. / Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages. In: Biotechnology Letters. 2006 ; Vol. 28, No. 11. pp. 815-821.
@article{22dc151d5bb84466b8fd2f445564372f,
title = "Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages",
abstract = "Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.",
keywords = "Chitosan, Gene transfer, Macrophage, Mannose, Plasmid DNA, Receptor-mediated endocytosis",
author = "Mayu Hashimoto and Minoru Morimoto and Hiroyuki Saimoto and Yoshihiro Shigemasa and Hironobu Yanagie and Masazumi Eriguchi and Toshinori Sato",
year = "2006",
month = "6",
doi = "10.1007/s10529-006-9006-x",
language = "English",
volume = "28",
pages = "815--821",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "11",

}

TY - JOUR

T1 - Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

AU - Hashimoto, Mayu

AU - Morimoto, Minoru

AU - Saimoto, Hiroyuki

AU - Shigemasa, Yoshihiro

AU - Yanagie, Hironobu

AU - Eriguchi, Masazumi

AU - Sato, Toshinori

PY - 2006/6

Y1 - 2006/6

N2 - Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

AB - Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

KW - Chitosan

KW - Gene transfer

KW - Macrophage

KW - Mannose

KW - Plasmid DNA

KW - Receptor-mediated endocytosis

UR - http://www.scopus.com/inward/record.url?scp=33745246316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745246316&partnerID=8YFLogxK

U2 - 10.1007/s10529-006-9006-x

DO - 10.1007/s10529-006-9006-x

M3 - Article

C2 - 16786247

AN - SCOPUS:33745246316

VL - 28

SP - 815

EP - 821

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 11

ER -