Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages

Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie, Masazumi Eriguchi, Toshinori Sato

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Chitosan is a biodegradable and biocompatible polymer and is useful as a non-viral vector for gene delivery. In order to deliver pDNA/chitosan complex into macrophages expressing a mannose receptor, mannose-modified chitosan (man-chitosan) was employed. The cellular uptake of pDNA/man-chitosan complexes through mannose recognition was then observed. The pDNA/man-chitosan complexes showed no significant cytotoxicity in mouse peritoneal macrophages, while pDNA/man-PEI complexes showed strong cytotoxicity. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes in mouse peritoneal macrophages. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes. Mannose receptor-mediated gene transfer thus enhances the transfection efficiency of pDNA/chitosan complexes.

Original languageEnglish
Pages (from-to)815-821
Number of pages7
JournalBiotechnology Letters
Volume28
Issue number11
DOIs
Publication statusPublished - 2006 Jun 1

Keywords

  • Chitosan
  • Gene transfer
  • Macrophage
  • Mannose
  • Plasmid DNA
  • Receptor-mediated endocytosis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Hashimoto, M., Morimoto, M., Saimoto, H., Shigemasa, Y., Yanagie, H., Eriguchi, M., & Sato, T. (2006). Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages. Biotechnology Letters, 28(11), 815-821. https://doi.org/10.1007/s10529-006-9006-x