Gene transfer into macrophage using chitosan nanoparticle modified with mannose

Mayu Hashimoto; Minoru Morimoto; Hiroyuki Saimoto; Yoshihiro Shigemasa; Hironobu Yanagie; Toshinori Sato

Gene transfer into macrophage using chitosan nanoparticle modified with mannose

Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie, Toshinori Sato

Department of Biosciences and Informatics

Research output: Contribution to conference › Paper › peer-review

Abstract

Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Chitosan
Gene transfer
Mannose
Receptor-mediated endocytosis

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Gene transfer into macrophage using chitosan nanoparticle modified with mannose",

abstract = "Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.",

keywords = "Chitosan, Gene transfer, Mannose, Receptor-mediated endocytosis",

author = "Mayu Hashimoto and Minoru Morimoto and Hiroyuki Saimoto and Yoshihiro Shigemasa and Hironobu Yanagie and Toshinori Sato",

year = "2005",

month = dec,

day = "1",

language = "English",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Gene transfer into macrophage using chitosan nanoparticle modified with mannose

AU - Hashimoto, Mayu

AU - Morimoto, Minoru

AU - Saimoto, Hiroyuki

AU - Shigemasa, Yoshihiro

AU - Yanagie, Hironobu

AU - Sato, Toshinori

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.

AB - Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.

KW - Chitosan

KW - Gene transfer

KW - Mannose

KW - Receptor-mediated endocytosis

UR - http://www.scopus.com/inward/record.url?scp=33645640242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645640242&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645640242

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Gene transfer into macrophage using chitosan nanoparticle modified with mannose

Abstract

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Keywords

ASJC Scopus subject areas

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