Gene transfer into macrophage using chitosan nanoparticle modified with mannose

Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie, Toshinori Sato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages2144
Number of pages1
Volume54
Edition1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Fingerprint

Gene transfer
Macrophages
Chitosan
Nanoparticles
Assays
DNA
Genes

Keywords

  • Chitosan
  • Gene transfer
  • Mannose
  • Receptor-mediated endocytosis

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Hashimoto, M., Morimoto, M., Saimoto, H., Shigemasa, Y., Yanagie, H., & Sato, T. (2005). Gene transfer into macrophage using chitosan nanoparticle modified with mannose. In Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2144)

Gene transfer into macrophage using chitosan nanoparticle modified with mannose. / Hashimoto, Mayu; Morimoto, Minoru; Saimoto, Hiroyuki; Shigemasa, Yoshihiro; Yanagie, Hironobu; Sato, Toshinori.

Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2144.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hashimoto, M, Morimoto, M, Saimoto, H, Shigemasa, Y, Yanagie, H & Sato, T 2005, Gene transfer into macrophage using chitosan nanoparticle modified with mannose. in Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2144, 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 05/5/25.
Hashimoto M, Morimoto M, Saimoto H, Shigemasa Y, Yanagie H, Sato T. Gene transfer into macrophage using chitosan nanoparticle modified with mannose. In Polymer Preprints, Japan. 1 ed. Vol. 54. 2005. p. 2144
Hashimoto, Mayu ; Morimoto, Minoru ; Saimoto, Hiroyuki ; Shigemasa, Yoshihiro ; Yanagie, Hironobu ; Sato, Toshinori. / Gene transfer into macrophage using chitosan nanoparticle modified with mannose. Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2144
@inproceedings{d7526395f88049f2bf48d07e77cbd802,
title = "Gene transfer into macrophage using chitosan nanoparticle modified with mannose",
abstract = "Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.",
keywords = "Chitosan, Gene transfer, Mannose, Receptor-mediated endocytosis",
author = "Mayu Hashimoto and Minoru Morimoto and Hiroyuki Saimoto and Yoshihiro Shigemasa and Hironobu Yanagie and Toshinori Sato",
year = "2005",
language = "English",
volume = "54",
pages = "2144",
booktitle = "Polymer Preprints, Japan",
edition = "1",

}

TY - GEN

T1 - Gene transfer into macrophage using chitosan nanoparticle modified with mannose

AU - Hashimoto, Mayu

AU - Morimoto, Minoru

AU - Saimoto, Hiroyuki

AU - Shigemasa, Yoshihiro

AU - Yanagie, Hironobu

AU - Sato, Toshinori

PY - 2005

Y1 - 2005

N2 - Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.

AB - Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.

KW - Chitosan

KW - Gene transfer

KW - Mannose

KW - Receptor-mediated endocytosis

UR - http://www.scopus.com/inward/record.url?scp=33645640242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645640242&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:33645640242

VL - 54

SP - 2144

BT - Polymer Preprints, Japan

ER -