Gene transfer into macrophage using chitosan nanoparticle modified with mannose

Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie, Toshinori Sato

Research output: Contribution to conferencePaper

Abstract

Mannose-modified chitosan was synthesized for receptor-mediated gene delivery to macrophage. In this study we evaluated the efficiency of the cellular uptake and transfection of chitosan/pDNA complexes. Mannosylation to chitosan enhanced the cellular uptake and transfection efficiency. Inhibition assay with mannan indicated mannose-modified chitosan/DNA complexes interacted with mannose receptor on macrophages. Moreover, the effect for activating of macrophages by chitosan/pDNA complexes was also evaluated. The production of NO was promoted by the chitosan/pDNA complexes.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Keywords

  • Chitosan
  • Gene transfer
  • Mannose
  • Receptor-mediated endocytosis

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Hashimoto, M., Morimoto, M., Saimoto, H., Shigemasa, Y., Yanagie, H., & Sato, T. (2005). Gene transfer into macrophage using chitosan nanoparticle modified with mannose. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.