Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa, Makoto Ogura, Shunichi Sato, Hitoshi Wakisaka, Hiroshi Ashida, Maki Uenoyama, Yoshinori Masaki, Minoru Obara

    Research output: Contribution to journalArticlepeer-review

    61 Citations (Scopus)

    Abstract

    Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (∼43°C) was effective in improving the transfection efficiency.

    Original languageEnglish
    Pages (from-to)1227-1229
    Number of pages3
    JournalOptics Letters
    Volume29
    Issue number11
    DOIs
    Publication statusPublished - 2004 Jun 1

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics

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