Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves

Mitsuhiro Terakawa, Shunichi Sato, Daizoh Saitoh, Makoto Hasegawa, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We successfully delivered a therapeutic vector construct, which carries hepatocyte growth factor (HGF) gene, to rat skin in vivo. After HGF expression vector had been intradermally injected to rat skin, LISWs were generated by irradiating the laser target put on the rat skin with nanosecond pulses from the second harmonics (532 nm) of a Q-switched Nd:YAG laser. Concentration of HGF protein increased by a factor of four by the application of LISWs when compared with that of control samples without LISW application. We also investigated the effects of LISWs on the integrity of plasmid DNA.

Original languageEnglish
Title of host publicationHigh-Power Laser Ablation VI
DOIs
Publication statusPublished - 2006 Aug 23
EventHigh-Power Laser Ablation VI - Taos, NM, United States
Duration: 2006 May 72006 May 12

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6261 II
ISSN (Print)0277-786X

Other

OtherHigh-Power Laser Ablation VI
CountryUnited States
CityTaos, NM
Period06/5/706/5/12

Keywords

  • Electrophoresis
  • Gene therapy
  • Gene transfer
  • HGF
  • Laser
  • Plasmid DNA
  • Skin
  • Stress wave

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Terakawa, M., Sato, S., Saitoh, D., Hasegawa, M., Ashida, H., Okano, H., & Obara, M. (2006). Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves. In High-Power Laser Ablation VI [626133] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6261 II). https://doi.org/10.1117/12.668965