Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves

Mitsuhiro Terakawa, Shunichi Sato, Daizoh Saitoh, Makoto Hasegawa, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We successfully delivered a therapeutic vector construct, which carries hepatocyte growth factor (HGF) gene, to rat skin in vivo. After HGF expression vector had been intradermally injected to rat skin, LISWs were generated by irradiating the laser target put on the rat skin with nanosecond pulses from the second harmonics (532 nm) of a Q-switched Nd:YAG laser. Concentration of HGF protein increased by a factor of four by the application of LISWs when compared with that of control samples without LISW application. We also investigated the effects of LISWs on the integrity of plasmid DNA.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume6261 II
DOIs
Publication statusPublished - 2006
EventHigh-Power Laser Ablation VI - Taos, NM, United States
Duration: 2006 May 72006 May 12

Other

OtherHigh-Power Laser Ablation VI
CountryUnited States
CityTaos, NM
Period06/5/706/5/12

Fingerprint

Gene transfer
stress waves
Pulsed lasers
genes
Rats
pulsed lasers
Skin
rats
Lasers
laser targets
plasmids
DNA
Q switched lasers
Genes
integrity
Proteins
YAG lasers
deoxyribonucleic acid
proteins
harmonics

Keywords

  • Electrophoresis
  • Gene therapy
  • Gene transfer
  • HGF
  • Laser
  • Plasmid DNA
  • Skin
  • Stress wave

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Terakawa, M., Sato, S., Saitoh, D., Hasegawa, M., Ashida, H., Okano, H., & Obara, M. (2006). Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 6261 II). [626133] https://doi.org/10.1117/12.668965

Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves. / Terakawa, Mitsuhiro; Sato, Shunichi; Saitoh, Daizoh; Hasegawa, Makoto; Ashida, Hiroshi; Okano, Hideyuki; Obara, Minoru.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6261 II 2006. 626133.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Terakawa, M, Sato, S, Saitoh, D, Hasegawa, M, Ashida, H, Okano, H & Obara, M 2006, Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 6261 II, 626133, High-Power Laser Ablation VI, Taos, NM, United States, 06/5/7. https://doi.org/10.1117/12.668965
Terakawa M, Sato S, Saitoh D, Hasegawa M, Ashida H, Okano H et al. Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6261 II. 2006. 626133 https://doi.org/10.1117/12.668965
Terakawa, Mitsuhiro ; Sato, Shunichi ; Saitoh, Daizoh ; Hasegawa, Makoto ; Ashida, Hiroshi ; Okano, Hideyuki ; Obara, Minoru. / Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6261 II 2006.
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