TY - JOUR
T1 - Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors
AU - Nakatake, Yuki
AU - Ko, Shigeru
AU - Sharov, Alexei A.
AU - Wakabayashi, Shunichi
AU - Murakami, Miyako
AU - Sakota, Miki
AU - Chikazawa, Nana
AU - Ookura, Chiaki
AU - Sato, Saeko
AU - Ito, Noriko
AU - Ishikawa-Hirayama, Madoka
AU - Mak, Siu Shan
AU - Jakt, Lars Martin
AU - Ueno, Tomoo
AU - Hiratsuka, Ken
AU - Matsushita, Misako
AU - Goparaju, Sravan Kumar
AU - Akiyama, Tomohiko
AU - Ishiguro, Kei ichiro
AU - Oda, Mayumi
AU - Gouda, Norio
AU - Umezawa, Akihiro
AU - Akutsu, Hidenori
AU - Nishimura, Kunihiro
AU - Matoba, Ryo
AU - Ohara, Osamu
AU - Ko, Minoru S.H.
N1 - Funding Information:
We thank all laboratory members for discussion and helpful suggestions; Motohiko Tanino, Yukari Ikeda, Ken Nonaka, and Hiroshi Iijima (DNA Chip) for their technical help in RNA-seq and microarray data analysis; Masakazu Machida (NrichD) for help with the SEES3 hESC line; and Utsumi Noriko (Kazusa DNA Research Institute) for handling human ORFs. This work was mainly supported by the CREST program from the Japan Science and Technology Agency (JST, grant number JPMJCR12W4 ). This work was supported in part by the Keio University Medical Science Fund – The Mitsunada Sakaguchi Laboratory and the Collaborative Research Resources, School of Medicine, Keio University and Japan Agency for Medical Research and Development (AMED, grant number 17bm0404012h0005 ).
Funding Information:
We thank all laboratory members for discussion and helpful suggestions; Motohiko Tanino, Yukari Ikeda, Ken Nonaka, and Hiroshi Iijima (DNA Chip) for their technical help in RNA-seq and microarray data analysis; Masakazu Machida (NrichD) for help with the SEES3 hESC line; and Utsumi Noriko (Kazusa DNA Research Institute) for handling human ORFs. This work was mainly supported by the CREST program from the Japan Science and Technology Agency (JST, grant number JPMJCR12W4). This work was supported in part by the Keio University Medical Science Fund ? The Mitsunada Sakaguchi Laboratory and the Collaborative Research Resources, School of Medicine, Keio University and Japan Agency for Medical Research and Development (AMED, grant number 17bm0404012h0005). Y.N. M. Murakami, M.S. N.C. C.O. S.S. N.I. M.I.H. S.S.M. L.M.J. T.U. K.H. M. Matsushita, S.K.G. T.A. K.-i.I. M.O. R.M. and O.O. carried out the experiments; Y.N. A.A.S. S.W. L.M.J. K.N. N.G. and M.S.H.K. carried out the data analysis; M.S.H.K. conceived the project; A.U. and H.A. provided a human ESC line (SEES3); O.O. provided most of the transgenes; S.B.H.K. H.A. K.N. R.M. O.O. and M.S.H.K. supervised the project; and Y.N. and M.S.H.K. wrote the manuscript with inputs from all authors. M.S.H.K. is a founder of Elixirgen Scientific, which receives a patent license from Keio University and commercializes transcription factor-based human pluripotent stem cell differentiation reagents. A.A.S. and N.C. are currently employed by Elixirgen Scientific.
Publisher Copyright:
© 2020 The Authors
PY - 2020/5/19
Y1 - 2020/5/19
N2 - Nakatake et al. establish 714 transgene-inducible human embryonic stem cell (hESC) lines and capture global transcriptome changes perturbed by the overexpression of these transgenes—mostly transcription factors (TFs). The analyses help in classifying TFs and identify sets of TFs that direct the differentiation of hESCs into distinct cell types.
AB - Nakatake et al. establish 714 transgene-inducible human embryonic stem cell (hESC) lines and capture global transcriptome changes perturbed by the overexpression of these transgenes—mostly transcription factors (TFs). The analyses help in classifying TFs and identify sets of TFs that direct the differentiation of hESCs into distinct cell types.
KW - cell differentiation
KW - conditional induction
KW - human embryonic stem cells
KW - transcription factors
KW - transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85084643670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084643670&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2020.107655
DO - 10.1016/j.celrep.2020.107655
M3 - Article
C2 - 32433964
AN - SCOPUS:85084643670
SN - 2211-1247
VL - 31
JO - Cell Reports
JF - Cell Reports
IS - 7
M1 - 107655
ER -