Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors

Yuhki Nakatake, Shigeru B.H. Ko, Alexei A. Sharov, Shunichi Wakabayashi, Miyako Murakami, Miki Sakota, Nana Chikazawa, Chiaki Ookura, Saeko Sato, Noriko Ito, Madoka Ishikawa-Hirayama, Siu Shan Mak, Lars Martin Jakt, Tomoo Ueno, Ken Hiratsuka, Misako Matsushita, Sravan Kumar Goparaju, Tomohiko Akiyama, Kei ichiro Ishiguro, Mayumi OdaNorio Gouda, Akihiro Umezawa, Hidenori Akutsu, Kunihiro Nishimura, Ryo Matoba, Osamu Ohara, Minoru S.H. Ko

Research output: Contribution to journalArticle

Abstract

Nakatake et al. establish 714 transgene-inducible human embryonic stem cell (hESC) lines and capture global transcriptome changes perturbed by the overexpression of these transgenes—mostly transcription factors (TFs). The analyses help in classifying TFs and identify sets of TFs that direct the differentiation of hESCs into distinct cell types.

Original languageEnglish
Article number107655
JournalCell Reports
Volume31
Issue number7
DOIs
Publication statusPublished - 2020 May 19

Keywords

  • cell differentiation
  • conditional induction
  • human embryonic stem cells
  • transcription factors
  • transcriptome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors'. Together they form a unique fingerprint.

  • Cite this

    Nakatake, Y., Ko, S. B. H., Sharov, A. A., Wakabayashi, S., Murakami, M., Sakota, M., Chikazawa, N., Ookura, C., Sato, S., Ito, N., Ishikawa-Hirayama, M., Mak, S. S., Jakt, L. M., Ueno, T., Hiratsuka, K., Matsushita, M., Goparaju, S. K., Akiyama, T., Ishiguro, K. I., ... Ko, M. S. H. (2020). Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors. Cell Reports, 31(7), [107655]. https://doi.org/10.1016/j.celrep.2020.107655