Generation of a bioengineered lacrimal gland by using the organ germ method

Masatoshi Hirayama, Kazuo Tsubota, Takashi Tsuji

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In organogenesis including lacrimal gland development, cell arrangement within a tissue plays an important role. The lacrimal gland develops from embryonic ocular surface epithelium through reciprocal epithelial and mesenchymal interaction, which is organized by interactive regulation of various pathways of signaling molecules. Current development of an in vitro three-dimensional cell manipulation procedure to generate a bioengineered organ germ, named as the organ germ method, has shown the regeneration of a histologically correct and fully functional bioengineered lacrimal gland after engraftment in vivo. This method demonstrated a possibility of lacrimal gland organ replacement to treat dry eye disease, which has been a public health problem leading reduction of visual function. Here, we describe protocols for lacrimal gland germ regeneration using the organ germ method and methods for analyzing the function of the bioengineered lacrimal gland after its transplantation in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-165
Number of pages13
Volume1597
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1597
ISSN (Print)10643745

Fingerprint

Lacrimal Apparatus
Regeneration
Organogenesis
Eye Diseases
Epithelium
Public Health
Transplantation

Keywords

  • Bioengineered lacrimal gland
  • Cell manipulation
  • Lacrimal gland germ
  • Organ germ method
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Hirayama, M., Tsubota, K., & Tsuji, T. (2017). Generation of a bioengineered lacrimal gland by using the organ germ method. In Methods in Molecular Biology (Vol. 1597, pp. 153-165). (Methods in Molecular Biology; Vol. 1597). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6949-4_11

Generation of a bioengineered lacrimal gland by using the organ germ method. / Hirayama, Masatoshi; Tsubota, Kazuo; Tsuji, Takashi.

Methods in Molecular Biology. Vol. 1597 Humana Press Inc., 2017. p. 153-165 (Methods in Molecular Biology; Vol. 1597).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hirayama, M, Tsubota, K & Tsuji, T 2017, Generation of a bioengineered lacrimal gland by using the organ germ method. in Methods in Molecular Biology. vol. 1597, Methods in Molecular Biology, vol. 1597, Humana Press Inc., pp. 153-165. https://doi.org/10.1007/978-1-4939-6949-4_11
Hirayama M, Tsubota K, Tsuji T. Generation of a bioengineered lacrimal gland by using the organ germ method. In Methods in Molecular Biology. Vol. 1597. Humana Press Inc. 2017. p. 153-165. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6949-4_11
Hirayama, Masatoshi ; Tsubota, Kazuo ; Tsuji, Takashi. / Generation of a bioengineered lacrimal gland by using the organ germ method. Methods in Molecular Biology. Vol. 1597 Humana Press Inc., 2017. pp. 153-165 (Methods in Molecular Biology).
@inbook{55a5d28525ad4498896ac5af08b8f030,
title = "Generation of a bioengineered lacrimal gland by using the organ germ method",
abstract = "In organogenesis including lacrimal gland development, cell arrangement within a tissue plays an important role. The lacrimal gland develops from embryonic ocular surface epithelium through reciprocal epithelial and mesenchymal interaction, which is organized by interactive regulation of various pathways of signaling molecules. Current development of an in vitro three-dimensional cell manipulation procedure to generate a bioengineered organ germ, named as the organ germ method, has shown the regeneration of a histologically correct and fully functional bioengineered lacrimal gland after engraftment in vivo. This method demonstrated a possibility of lacrimal gland organ replacement to treat dry eye disease, which has been a public health problem leading reduction of visual function. Here, we describe protocols for lacrimal gland germ regeneration using the organ germ method and methods for analyzing the function of the bioengineered lacrimal gland after its transplantation in vivo.",
keywords = "Bioengineered lacrimal gland, Cell manipulation, Lacrimal gland germ, Organ germ method, Transplantation",
author = "Masatoshi Hirayama and Kazuo Tsubota and Takashi Tsuji",
year = "2017",
doi = "10.1007/978-1-4939-6949-4_11",
language = "English",
volume = "1597",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "153--165",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Generation of a bioengineered lacrimal gland by using the organ germ method

AU - Hirayama, Masatoshi

AU - Tsubota, Kazuo

AU - Tsuji, Takashi

PY - 2017

Y1 - 2017

N2 - In organogenesis including lacrimal gland development, cell arrangement within a tissue plays an important role. The lacrimal gland develops from embryonic ocular surface epithelium through reciprocal epithelial and mesenchymal interaction, which is organized by interactive regulation of various pathways of signaling molecules. Current development of an in vitro three-dimensional cell manipulation procedure to generate a bioengineered organ germ, named as the organ germ method, has shown the regeneration of a histologically correct and fully functional bioengineered lacrimal gland after engraftment in vivo. This method demonstrated a possibility of lacrimal gland organ replacement to treat dry eye disease, which has been a public health problem leading reduction of visual function. Here, we describe protocols for lacrimal gland germ regeneration using the organ germ method and methods for analyzing the function of the bioengineered lacrimal gland after its transplantation in vivo.

AB - In organogenesis including lacrimal gland development, cell arrangement within a tissue plays an important role. The lacrimal gland develops from embryonic ocular surface epithelium through reciprocal epithelial and mesenchymal interaction, which is organized by interactive regulation of various pathways of signaling molecules. Current development of an in vitro three-dimensional cell manipulation procedure to generate a bioengineered organ germ, named as the organ germ method, has shown the regeneration of a histologically correct and fully functional bioengineered lacrimal gland after engraftment in vivo. This method demonstrated a possibility of lacrimal gland organ replacement to treat dry eye disease, which has been a public health problem leading reduction of visual function. Here, we describe protocols for lacrimal gland germ regeneration using the organ germ method and methods for analyzing the function of the bioengineered lacrimal gland after its transplantation in vivo.

KW - Bioengineered lacrimal gland

KW - Cell manipulation

KW - Lacrimal gland germ

KW - Organ germ method

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=85016940851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016940851&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6949-4_11

DO - 10.1007/978-1-4939-6949-4_11

M3 - Chapter

C2 - 28361316

AN - SCOPUS:85016940851

VL - 1597

T3 - Methods in Molecular Biology

SP - 153

EP - 165

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -