Generation of a bioengineered lacrimal gland by using the organ germ method

Masatoshi Hirayama, Kazuo Tsubota, Takashi Tsuji

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In organogenesis including lacrimal gland development, cell arrangement within a tissue plays an important role. The lacrimal gland develops from embryonic ocular surface epithelium through reciprocal epithelial and mesenchymal interaction, which is organized by interactive regulation of various pathways of signaling molecules. Current development of an in vitro three-dimensional cell manipulation procedure to generate a bioengineered organ germ, named as the organ germ method, has shown the regeneration of a histologically correct and fully functional bioengineered lacrimal gland after engraftment in vivo. This method demonstrated a possibility of lacrimal gland organ replacement to treat dry eye disease, which has been a public health problem leading reduction of visual function. Here, we describe protocols for lacrimal gland germ regeneration using the organ germ method and methods for analyzing the function of the bioengineered lacrimal gland after its transplantation in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages153-165
Number of pages13
Volume1597
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1597
ISSN (Print)10643745

Keywords

  • Bioengineered lacrimal gland
  • Cell manipulation
  • Lacrimal gland germ
  • Organ germ method
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Hirayama, M., Tsubota, K., & Tsuji, T. (2017). Generation of a bioengineered lacrimal gland by using the organ germ method. In Methods in Molecular Biology (Vol. 1597, pp. 153-165). (Methods in Molecular Biology; Vol. 1597). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6949-4_11