Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele

Sho Yoshimatsu, Rei Murakami, Tsukika Sato, Tsubasa Saeki, Masafumi Yamamoto, Erika Sasaki, Toshiaki Noce, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review

Abstract

POU class 5 homeobox 1 (POU5F1, also known as OCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. Homozygous OCT4 knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus, DDX4 (VASA).

Original languageEnglish
Article number102308
JournalStem Cell Research
Volume53
DOIs
Publication statusPublished - 2021 May

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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