Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

Sho Yoshimatsu, Tsukika Sato, Masafumi Yamamoto, Erika Sasaki, Mayutaka Nakajima, Mari Nakamura, Seiji Shiozawa, Toshiaki Noce, Hideyuki Okano

Research output: Contribution to journalArticle

Abstract

BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.

Original languageEnglish
Article number101740
JournalStem Cell Research
Volume44
DOIs
Publication statusPublished - 2020 Apr

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ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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