Generation of a monoclonal antibody that induces apoptosis of hematopoietic cells

Hiroyuki Mio, Mitsuo Satoh, Sachiko Yokokawa, Hitoshi Nishio, Toshio Suda, Tatsuya Tamaoki

Research output: Contribution to journalArticle

Abstract

Here, we developed anti-murine myeloid cell (NFS-60 cell) monoclonal antibodies in order to characterize the further mechanism of cell-to-cell interaction between the stromal cells and the hematopoietic progenitor cells. The established antibody, designated mAb UNF, inhibited proliferation of NFS-60 cells and induced apoptosis. Incubation of NFS-60 cells with mAb UNF initiated the cell aggregation within 3 hours. This phenomena did not require newly synthesized proteins, since the pretreatment of 1 mM cycloheximide failed to prevent the agglutination. Fifty-eight percent of mouse hematopoietic stem cells, Lin-, c-Kit+, Sca-1+ bone marrow cells, were shown to express UNF antigen. The glycolipid prepared from NFS-60 cells was specifically immunostained by mAb UNF.

Original languageEnglish
Pages (from-to)727-731
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume243
Issue number3
DOIs
Publication statusPublished - 1998 Feb 24

Keywords

  • Apoptosis
  • Hematopoietic stem cells
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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