Generation of B lymphocytes from a single hemopoietic progenitor cell in vitro

A. Ohara, T. Suda, N. Tokuyama, J. Suda, K. I. Nakayama, Y. Miura, S. I. Nishikawa, H. Nakauchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The first stages of the pathway by which lymphocytes differentiate from hemopoietic stem cells were studied at a clonal level. When 211 interleukin 3 (IL-3)-induced blast colonies shown to be capable of differentiating into a variety of hemopoietic cells were individually transferred into wells containing a monolayer of stromal cells, growth in granulocyte, macrophage, megakaryocyte, or mast cell lineages was observed in 192 wells. In seven of these 192 wells, lymphoid cell growth also was seen. The lymphoid cells were proved to be B lymphocytes by phenotype and immunoglobulin gene rearrangement analyses and by demonstration of surface expression of IgM. The clonal origin of myeloid and B lymphocyte lineage cells was further confirmed by the generation of both myeloid and B lymphoid cells in the same well following FACS clone-sorting of IL-3 induced blast cells. These results provide in vitro evidence that cells of B lymphoid and myeloid lineage can originate clonally from single primitive hemopoietic stem cells.

Original languageEnglish
Pages (from-to)703-709
Number of pages7
JournalInternational Immunology
Volume3
Issue number7
Publication statusPublished - 1991

Fingerprint

B-Lymphocytes
Stem Cells
Lymphocytes
Interleukin-3
Immunoglobulin Genes
Megakaryocytes
Gene Rearrangement
Cell Lineage
Myeloid Cells
Stromal Cells
Growth
Granulocytes
Mast Cells
Immunoglobulin M
Clone Cells
Macrophages
In Vitro Techniques
Phenotype

Keywords

  • Clone-sorting
  • FACS
  • Hemopoietic stem cell
  • Stroma cells

ASJC Scopus subject areas

  • Immunology

Cite this

Ohara, A., Suda, T., Tokuyama, N., Suda, J., Nakayama, K. I., Miura, Y., ... Nakauchi, H. (1991). Generation of B lymphocytes from a single hemopoietic progenitor cell in vitro. International Immunology, 3(7), 703-709.

Generation of B lymphocytes from a single hemopoietic progenitor cell in vitro. / Ohara, A.; Suda, T.; Tokuyama, N.; Suda, J.; Nakayama, K. I.; Miura, Y.; Nishikawa, S. I.; Nakauchi, H.

In: International Immunology, Vol. 3, No. 7, 1991, p. 703-709.

Research output: Contribution to journalArticle

Ohara, A, Suda, T, Tokuyama, N, Suda, J, Nakayama, KI, Miura, Y, Nishikawa, SI & Nakauchi, H 1991, 'Generation of B lymphocytes from a single hemopoietic progenitor cell in vitro', International Immunology, vol. 3, no. 7, pp. 703-709.
Ohara A, Suda T, Tokuyama N, Suda J, Nakayama KI, Miura Y et al. Generation of B lymphocytes from a single hemopoietic progenitor cell in vitro. International Immunology. 1991;3(7):703-709.
Ohara, A. ; Suda, T. ; Tokuyama, N. ; Suda, J. ; Nakayama, K. I. ; Miura, Y. ; Nishikawa, S. I. ; Nakauchi, H. / Generation of B lymphocytes from a single hemopoietic progenitor cell in vitro. In: International Immunology. 1991 ; Vol. 3, No. 7. pp. 703-709.
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