Generation of megakaryocytes and platelets from preadipocyte cell line 3T3-L1, but not the parent cell line 3T3, in vitro

Yumiko Matsubara, Hidenori Suzuki, Yasuo Ikeda, Mitsuru Murata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Platelets are produced from megakaryocytes (MKs), although the pathway leading from stem cells to MK lineages are not yet fully understood. Recently, we reported to obtain abundant MKs and platelets from human subcutaneous adipose tissues. Adipose tissues contain various cell types, most of which are lineage cells from mesenchymal or adipocyte-derived stem cells, distinct from hematopoietic cells. To identify the cells responsible for the differentiation MK lineages in adipose tissues, this study examined whether the preadipocyte cell line 3T3-L1 and fibroblast cell line 3T3 differentiated into MK lineages in vitro. Cells were cultured in megakaryocyte lineage induction medium. By day 4, most of 3T3 cell-derived cells leaded to cell death. In contrast, 3T3-L1-derived cells on days 8 showed to have typical characterizations of MK lineages in analyses for specific marker, DNA ploidy, transmission electro micrograph. 3T3-L1-derived platelet-sized cells on day 12 could be stimulated by ADP and PAR4-activating peptide. This study clearly shows in vitro differentiation from 3T3-L1 cells, not from 3T3 cells, into MK lineages.

Original languageEnglish
Pages (from-to)796-800
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume402
Issue number4
DOIs
Publication statusPublished - 2010 Nov 26

Fingerprint

Megakaryocytes
Platelets
Blood Platelets
Cells
Tissue
Stem cells
Cell Line
3T3-L1 Cells
Cell death
Fibroblasts
3T3 Cells
Adenosine Diphosphate
Adipose Tissue
Peptides
DNA
In Vitro Techniques
Ploidies
Subcutaneous Fat
Hematopoietic Stem Cells
Genetic Markers

Keywords

  • 3T3 cells
  • 3T3-L1 cells
  • Megakaryocytes
  • Platelets
  • Preadipocytes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Generation of megakaryocytes and platelets from preadipocyte cell line 3T3-L1, but not the parent cell line 3T3, in vitro. / Matsubara, Yumiko; Suzuki, Hidenori; Ikeda, Yasuo; Murata, Mitsuru.

In: Biochemical and Biophysical Research Communications, Vol. 402, No. 4, 26.11.2010, p. 796-800.

Research output: Contribution to journalArticle

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