Generation of mice deficient in RNA-binding motif protein 3 (RBM3) and characterization of its role in innate immune responses and cell growth

Atsushi Matsuda, Masahiro Ogawa, Hideyuki Yanai, Daiji Naka, Ayana Goto, Tomoka Ao, Yuji Tanno, Kiyoshi Takeda, Yoshinori Watanabe, Kenya Honda, Tadatsugu Taniguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The activation of innate immune responses is critical to host defense against microbial infections, wherein nucleic acid-sensing pattern recognition receptors recognize DNA or RNA from viruses or bacteria and activate downstream signaling pathways. In a search for new DNA-sensing molecules that regulate innate immune responses, we identified RNA-binding motif protein 3 (RBM3), whose role has been implicated in the regulation of cell growth. In this study, we generated Rbm3-deficient (Rbm3-/-) mice to study the role of RBM3 in immune responses and cell growth. Despite evidence for its interaction with immunogenic DNA in a cell, no overt phenotypic abnormalities were found in cells from Rbm3-/- mice for the DNA-mediated induction of cytokine genes. Interestingly, however, Rbm3-/- mouse embryonic fibroblasts (MEFs) showed poorer proliferation rates as compared to control MEFs. Further cell cycle analysis revealed that Rbm3-/- MEFs have markedly increased number of G2-phase cells, suggesting a hitherto unknown role of RBM3 in the G2-phase control. Thus, these mutant mice and cells may provide new tools with which to study the mechanisms underlying the regulation of cell cycle and oncogenesis.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume411
Issue number1
DOIs
Publication statusPublished - 2011 Jul 22
Externally publishedYes

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Keywords

  • Cell growth
  • G2/M
  • Innate immunity
  • PRRs
  • RBM3

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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