Generation of mouse ES cell lines engineered for the forced induction of transcription factors

Lina S. Correa-Cerro, Yulan Piao, Alexei A. Sharov, Akira Nishiyama, Jean S. Cadet, Hong Yu, Lioudmila V. Sharova, Li Xin, Hien G. Hoang, Marshall Thomas, Yong Qian, Dawood B. Dudekula, Emily Meyers, Bernard Y. Binder, Gregory Mowrer, Uwem Bassey, Dan L. Longo, David Schlessinger, Minoru Ko

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Abstract

Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.

Original languageEnglish
Article number167
JournalScientific Reports
Volume1
DOIs
Publication statusPublished - 2011
Externally publishedYes

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Correa-Cerro, L. S., Piao, Y., Sharov, A. A., Nishiyama, A., Cadet, J. S., Yu, H., Sharova, L. V., Xin, L., Hoang, H. G., Thomas, M., Qian, Y., Dudekula, D. B., Meyers, E., Binder, B. Y., Mowrer, G., Bassey, U., Longo, D. L., Schlessinger, D., & Ko, M. (2011). Generation of mouse ES cell lines engineered for the forced induction of transcription factors. Scientific Reports, 1, [167]. https://doi.org/10.1038/srep00167