@inbook{9d885644dabf4d5d8f849b267dfd7f98,
title = "Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System",
abstract = "Transposable elements (TEs) constitute a large proportion of the genome in multiple organisms. Therefore, anti-transposable element machineries are essential to maintain genomic integrity. PIWI-interacting RNAs (piRNAs) are a major force to repress TEs in Drosophila ovaries. Ovarian somatic cells (OSC), in which nuclear piRNA regulation is functional, have been used for research on piRNA pathway as a cell culture system to elucidate the molecular mechanisms underlying the piRNA pathway. Analysis of piRNA pathway using a reporter system to monitor the gene regulation or overexpression of specific genes would be a powerful approach. Here, we present the technical protocol to establish stable cell lines using the piggyBac system, adopted for OSCs. This easy, consistent, and timesaving protocol may accelerate research on the piRNA pathway.",
keywords = "Ovarian somatic cell, PIWI, Stable line, piRNA pathway, piggyBac",
author = "Chikara Takeuchi and Kensaku Murano and Mitsuru Ishikawa and Hideyuki Okano and Iwasaki, {Yuka W.}",
note = "Funding Information: We thank Dr. Haruhiko Siomi for the critical reading of the manuscript. OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast, used for construction of donor plasmid, was a kind gift from Dr. Julius Brennecke (Addgene plasmid # 128010; http://n2t.net/ addgene:128010; RRID:Addgene_128010). YWI is supported by funding from JSPS KAKENHI Grant Numbers 22H02547, 21H00259 and 18H02421, JST PRESTO Grant Number JPMJPR20E2. KM is supported by funding from JSPS KAKENHI Grant Number 20H03439. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2380-0_9",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "143--153",
booktitle = "Methods in Molecular Biology",
}