Generation of transgenic non-human primates with germline transmission

Erika Sasaki, Hiroshi Suemizu, Akiko Shimada, Kisaburo Hanazawa, Ryo Oiwa, Michiko Kamioka, Ikuo Tomioka, Yusuke Sotomaru, Reiko Hirakawa, Tomoo Eto, Seiji Shiozawa, Takuji Maeda, Mamoru Ito, Ryoji Ito, Chika Kito, Chie Yagihashi, Kenji Kawai, Hiroyuki Miyoshi, Yoshikuni Tanioka, Norikazu Tamaoki & 3 others Sonoko Habu, Hideyuki Okano, Tatsuji Nomura

Research output: Contribution to journalArticle

412 Citations (Scopus)

Abstract

The common marmoset (Callithrix jacchus) is increasingly attractive for use as a non-human primate animal model in biomedical research. It has a relatively high reproduction rate for a primate, making it potentially suitable for transgenic modification. Although several attempts have been made to produce non-human transgenic primates, transgene expression in the somatic tissues of live infants has not been demonstrated by objective analyses such as polymerase chain reaction with reverse transcription or western blots. Here we show that the injection of a self-inactivating lentiviral vector in sucrose solution into marmoset embryos results in transgenic common marmosets that expressed the transgene in several organs. Notably, we achieved germline transmission of the transgene, and the transgenic offspring developed normally. The successful creation of transgenic marmosets provides a new animal model for human disease that has the great advantage of a close genetic relationship with humans. This model will be valuable to many fields of biomedical research.

Original languageEnglish
Pages (from-to)523-527
Number of pages5
JournalNature
Volume459
Issue number7246
DOIs
Publication statusPublished - 2009 May 28

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Callithrix
Primates
Transgenes
Biomedical Research
Animal Disease Models
Reverse Transcription
Reproduction
Sucrose
Embryonic Structures
Animal Models
Western Blotting
Polymerase Chain Reaction
Injections

ASJC Scopus subject areas

  • General

Cite this

Sasaki, E., Suemizu, H., Shimada, A., Hanazawa, K., Oiwa, R., Kamioka, M., ... Nomura, T. (2009). Generation of transgenic non-human primates with germline transmission. Nature, 459(7246), 523-527. https://doi.org/10.1038/nature08090

Generation of transgenic non-human primates with germline transmission. / Sasaki, Erika; Suemizu, Hiroshi; Shimada, Akiko; Hanazawa, Kisaburo; Oiwa, Ryo; Kamioka, Michiko; Tomioka, Ikuo; Sotomaru, Yusuke; Hirakawa, Reiko; Eto, Tomoo; Shiozawa, Seiji; Maeda, Takuji; Ito, Mamoru; Ito, Ryoji; Kito, Chika; Yagihashi, Chie; Kawai, Kenji; Miyoshi, Hiroyuki; Tanioka, Yoshikuni; Tamaoki, Norikazu; Habu, Sonoko; Okano, Hideyuki; Nomura, Tatsuji.

In: Nature, Vol. 459, No. 7246, 28.05.2009, p. 523-527.

Research output: Contribution to journalArticle

Sasaki, E, Suemizu, H, Shimada, A, Hanazawa, K, Oiwa, R, Kamioka, M, Tomioka, I, Sotomaru, Y, Hirakawa, R, Eto, T, Shiozawa, S, Maeda, T, Ito, M, Ito, R, Kito, C, Yagihashi, C, Kawai, K, Miyoshi, H, Tanioka, Y, Tamaoki, N, Habu, S, Okano, H & Nomura, T 2009, 'Generation of transgenic non-human primates with germline transmission', Nature, vol. 459, no. 7246, pp. 523-527. https://doi.org/10.1038/nature08090
Sasaki E, Suemizu H, Shimada A, Hanazawa K, Oiwa R, Kamioka M et al. Generation of transgenic non-human primates with germline transmission. Nature. 2009 May 28;459(7246):523-527. https://doi.org/10.1038/nature08090
Sasaki, Erika ; Suemizu, Hiroshi ; Shimada, Akiko ; Hanazawa, Kisaburo ; Oiwa, Ryo ; Kamioka, Michiko ; Tomioka, Ikuo ; Sotomaru, Yusuke ; Hirakawa, Reiko ; Eto, Tomoo ; Shiozawa, Seiji ; Maeda, Takuji ; Ito, Mamoru ; Ito, Ryoji ; Kito, Chika ; Yagihashi, Chie ; Kawai, Kenji ; Miyoshi, Hiroyuki ; Tanioka, Yoshikuni ; Tamaoki, Norikazu ; Habu, Sonoko ; Okano, Hideyuki ; Nomura, Tatsuji. / Generation of transgenic non-human primates with germline transmission. In: Nature. 2009 ; Vol. 459, No. 7246. pp. 523-527.
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